...Plasmid Collections Depositor Plasmid Collections Depositor Collections Addgene's repository contains 158,806...collections from depositors. To help you find what you're looking for, we've gathered our depositor collections...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression...

...Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid 68289 SepOTSθ...68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 SepOTSν Plasmid...Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ Plasmid ....Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep... Depositor Collections Rinehart Lab Phosphoprotein Reagents...188537 ) a better choice in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1...can be generated with either phosphoserine using SepOTSλ ( 68292 ) or serine using supD tRNA ( 68307 ) at...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...imaging (Perceval) A genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2)...

...Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events...they activate expression of the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various...Purification Controlled Expression Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular...promoters, their associated transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum...bacterium. Plasmids containing easily measurable reporter genes (eg. LacZ or Fluorescent Proteins ) under...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...

...Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, ...transcriptional regulation and bioluminescent reporters. Plasmid...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible... common choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors...-lives, making them useful as transcriptional reporters. However, they are not secreted, so cells must...CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle of the Firefly.... pKC-4.04, pKC-4.06 : Nonsense-mediated decay reporter and control plasmid utilizing Firefly luciferase...

...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...window) Jackson Labs - mouse line developer and repository, and CRO for centralized behavior scoring (Link... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...contributing your plasmids! To get started with your deposit, please click here ....

...shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor organized by a common...Collections Addgene Plasmid Collections Addgene's repository contains 158,806 plasmids . We've gathered our...our curated plasmid resources, kits, and depositor collections on one page to help you find what you're...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...common use. Click here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein...community that has established a common place for deposited plasmids. ASAP Discovery Consortium ARF GTPase...Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease research areas. ...

...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...Mammalian Pelczar 62732 Cre Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References Background...Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized...preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System...HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG...Alcudia 108639 fli1aCreERT2 Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 ...

...the community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse ...available at Addgene. Browse featured collections or depositing labs. Plasmid...Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks BioBricks Foundation...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones...genes can be inserted into this firefly luciferase reporter to test for their effects on protein production...

...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...previously we will reach out to the lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries...

... aren't in the repository? Help us widen our resources for the community by depositing your useful tools...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...bacteria, and more. Synthetic Biology : Browse our deposited tools and resources, or read our guide on assembly... reference page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast...Genome Engineering blog post to see how Addgene depositors are making this possible in a range of organisms...

...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...

... Depositor Collections Bikard Lab - CRISPR Repression Collection...David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays to modulate the ...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

... Addgene, which highlights CMC depositing labs and plasmids.... Depositor Collections Cell Migration Consortium...laboratories of the Cell Migration Consortium have deposited plasmids useful for cell migration research at...at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge , Harvard Medical School...

...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery method...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid... Depositor Collections Brzezinski Lab CRISPR Collection Brzezinski...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...