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We narrowed to 25 results for: aav vectors expressing gfp

Showing: 1 - 20 of 25 results

  1. Brain Initiative Collection

    Type
    Collection
    ...-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  2. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9-X1.1 ...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service...

  3. Retrograde AAV viral preps

    Type
    Collection
    ...Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part...Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent...Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx...Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA...58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Edward Boyden 59170 pAAV-Syn-Chronos-GFP Syn ...mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP...Cre expression Recombinases James M. Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged...

  4. Brain Armamentarium

    Type
    Collection
    ...Daigle James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden...Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic... Tasic Melina Fan 221463-AAV1 pAAV_BiPVe4_eGFP AAV construct expressing eGFP driven by chandelier cell-targeting... Viviana Gradinaru 213947-AAV1 pAAV_BiSSTe4_GCaMP6f AAV construct expressing GCaMP6f driven by SST interneuron-targeting...

  5. AAV Molecular Tools

    Type
    Collection
    ... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular... Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System...)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven... ID Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin...-inducible expression of EYFP 1 Viviana Gradinaru Tools for Affinity Purification These AAV encode tools...Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression System Activity...

  6. Viral Production

    Type
    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with ... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and ...

  8. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our...our control AAV that express fluorescent proteins under general promoters. These AAV can be used to compare...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9 AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive...83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell... PHP.eB, PHP.S Edward Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB...

  9. Serotype Testing AAV

    Type
    Collection
    ...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode ...the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description...Ready-to-use AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for...serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde. Viral.... These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...transient expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast...lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under the...-On vector expressing TetR from CMV promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On... rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG promoter. See...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of ...pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc...Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc ...Firefly CAG AAV expression of firefly luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...

  13. Adenovirus Plasmids

    Type
    Collection
    ... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of...regions present in pAd5-B6 ∆E3-GFP and pAd5-B7 . Bunz 179203 pAd5-B1-deltaE1-GFP Adenoviral A block for the...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal...Collection of adenovirus shuttle vectors and strains Viral...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...

  14. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...also expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also .... Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-...fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also ...Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 50 17 min...dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral...Mammalian Expression mEmerald-C1 - Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507...mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern -...pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...

  16. Recombinases AAV Preps

    Type
    Collection
    ...Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre...Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...rg* James M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP...Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore...with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory...from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ... pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6-gRNA 79145...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...based on: Your particular expression system , eg. Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101...using AAV vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...

  19. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral ...Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin.... Viral Vector Packaging Service AAV University of Florida Serotype Testing...viral production , these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71. PMID:...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their ...

  20. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our...pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn... our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control of ...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...
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