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We narrowed to 25 results for: lentiviral plasmid

Showing: 1 - 20 of 25 results
  1. Plasmids for Stem Cell Research

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    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent... Myc, and Brd3R from five separate lentiviral plasmids The acetyllysine reader BRD3R promotes human nuclear..., and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human...Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible system for direct reprogramming...
  2. Lentivirus Plasmids

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    ...safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express...Lentiviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset... Viral Plasmids Lentiviral Plasmids Lentiviral...Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description...Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732...Kasparov Return to top Popular Lentiviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G...Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All Viral Plasmids Viral...
  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...
  4. Luciferase Plasmid Collection

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    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... Plasmid Collections Luciferase...
  5. Kazuhiro Oka Lentiviral Vectors

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    ...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Content last reviewed: 15 August 2025...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor...
  6. SARS-CoV-2 Pseudotyped Virus

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    ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...
  7. Viral Production

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    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...
  8. Tetracycline Inducible Expression

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    .... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR...additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1 Lentiviral Tet-On vector...Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene...
  9. Mammalian RNAi Tools

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    ...viral delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid... Plasmid Collections Mammalian RNAi Mammalian shRNA...
  10. Lentiviral Prep Service

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    ...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...available in lentiviral format. Viral...repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...
  11. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile...genes and divided into multi-plasmid systems. These systems consist of a plasmid containing the genetic information...be delivered (transfer or shuttle plasmid) and one or more plasmids containing the necessary components...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...required genes are divided in the multi-plasmid system. Many viral plasmids are also self-inactivating, meaning...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...
  12. CRISPR Plasmids - Empty gRNA Vectors

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    ...-only plasmids are not limited to a specific CRISPR function, but require an additional plasmid containing...catalog for control gRNAs . All-in-one Plasmids (gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker...Marker PI Publication Return to top gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI...Publication Return to top Multiplex gRNA Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI... Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA plasmids, including: Multiplex...of other CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
  13. Plan Your Experiment

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    ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...
  14. Zhang Lab CRISPR Page

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    ... Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice...
  15. Neurodegeneration Research Collection

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    ...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Dev Cell. 2024) Image exocytosis with VAMP2/3 plasmids to study CNS myelination. (Lam et al. Nat Commun...Alzheimer's disease and related dementias with plasmids from the iPSC Neurodegenerative Disease Initiative...
  16. CRISPR Plasmids and Resources

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    ... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...
  17. Fluorescent Protein Guide: Empty Backbones

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    ...Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...
  18. CRISPR Guide

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    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...
  19. COVID-19 Resources

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    ... Plasmid Collections COVID-19 – Plasmids and Resources COVID-19 and Coronavirus Plasmids & Resources...Antibodies SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries...Brian Geiss Plasmids SARS-CoV-2 Plasmids Browse the tables below to find popular plasmids expressing genes...to top Other Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI ...for-profit entity, please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional...to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection – Addgene partnered with...-CoV and SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI Additional...
  20. CRISPR References and Information

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    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators...
Showing: 1 - 20 of 25 results