We narrowed to 26 results for: pET
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TypeCollection...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...
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Structural Genomics Consortium Plasmids
TypeCollection...cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC EF442785 Hexahistidine...26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL ... -
CRISPR Guide
TypeCollection...have been used for dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and...labeled proteins to the site. Chromosome-specific repetitive loci can be efficiently visualized in living ...multiple target sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., & Joung, J. .... Cell . 184 (9), 2503-2519.e17. PMID: 33838111 Peters, J. M., Koo, B., Patino, R., Heussler, G. E., Hearne... S., Silvis, M. R., Harden, M. M., Osadnik, H., Peters, J. E., Engel, J. N., Dutton, R. J., Grossman, ... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...facilitates screening by allowing a multichannel pipette to be used for the remainder of the steps in step...well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and ...clone present. Resuspend cells in 200 μl of media. Pipette mix to detach cells. Plate 100 μl each into two... -
Luciferase Plasmid Collection
TypeCollection... luciferase in the cytoplasm of mammalian cells Peter Cockerill 64784 pGL3-Basic-IRES Firefly Insertion... investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector... investigating regions controlling transcription Pete Stecha Return to Top Expression Constructs Luciferase... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction...publications, please acknowledge Arun Srivastava and cite Petrs-Silva, et al. 2011. Novel properties of tyrosine-mutant... -
Trimmer Lab NeuroMab Collection
TypeCollection...Anti-Keyhole limpet hemocyanin [N132A/12R] Keyhole limpet hemocyanin Giant keyhole limpet Mouse IgG2a ... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...Malate dehydrogenase CUREs Community (MCC). DeChenne-Peters et al. PLoS One. 2023 Mar 9;18(3):e0282170. PMID... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...academic community, the labs of Keith Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays... -
TALEN Guide
TypeCollection...TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug...