Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 91 results for: tro

Clear keywords
Showing: 31 - 60 of 91 results

  1. CRISPR Guide

    Type
    Collection
    ...provide a basic understanding of CRISPR biology, introduce the various applications of CRISPR, and help you...research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR and some of the... nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity ...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific...

  2. Plan Your Experiment

    Type
    Collection
    ...CRISPR Experiment Jump To: Plan Your Experiment Introduction Select Genetic Manipulation Design and Clone...generally perform by chemical transfection or electroporation. Expression can be transient, or you can generate... of Cas and gRNA. This method involves using in vitro transcription reactions to generate mature Cas mRNA... to target cells through microinjection or electroporation. The target cells translate the mRNA encoding...you directly combine purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You...delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA...Weissman, J. S. (2014). Genome-Scale CRISPR-Mediated control of gene repression and activation. Cell , 159 (...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...collections available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes...Reprogram astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...being adopted in the field. Read on for a brief introduction to the genetics associated with some of the ...Symptoms include changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty...neurons in the substantia nigra, one of the movement control centers in the brain. The cause of this neuron ...

  4. AAV Viral Preps

    Type
    Collection
    ...samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde...is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy .... Addgene’s comprehensive high-quality quality control process includes titering, purity assessment, sterility... Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...optogenetically controlled Cre/loxP system that enables precise temporal and spatial control of gene expression...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR...ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study...study the development and function of zebrafish astrocytes. Knock-in tagging - Michel Bagnat lab. Targeting...

  6. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...intracerebroventricular (ICV) delivery in newborns, and retro-orbital (RO) and stereotaxic (STX) delivery in adult...minBG-SYFP2-WPRE3-BGHpA AiP14470 AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic nucleus (VMH) Hypothalamus 230531...minBG-SYFP2-WPRE3-BGHpA AiP12558 AiE0390m_3xC2 SYFP2 Astrocytes Whole Brain 208163 pAAV-AiE0390m_3xC2-minBG-iCre...-iCre(R297T)-BGHpA AiP14801 AiE0390m_3xC2 Cre Astrocytes Whole Brain 208143 pAAV-AiE0390h_3xC2-minBG-SYFP2...minBG-SYFP2-WPRE3-BGHpA AiP13572 AiE0390h_3xC2 SYFP2 Astrocytes Whole Brain 208133 pAAV-AiE0387m-minBglobin-SYFP2...-SYFP2-WPRE3-BGHpA AiP12102 AiE0387m SYFP2 Astrocytes Whole Brain 208166 pAAV-AiE0387m-minBG-iCre(R297T...R297T)-BGHpA AiP13999 AiE0387m Cre Astrocytes Whole Brain 220643 pAAV-AiE2128m-minBG-SYFP2-WPRE3-BGHpA AiP1357...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7...promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis of TALEN mRNAs. Both 5’ and 3’ Xenopus...of the DNA-binding domain of a TALE, along with strong nuclear localisation tags and repress transcription... T7 promoter. Truncations were introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which ... included for generating TALEN mRNAs through in vitro transcription as an alternative. Based on transient...

  8. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Production and Quality Control Production Our production process and quality control, with examples of quality...production, purification, and comprehensive quality control. In-Stock Preps 1,000+ preps ready to ship immediately...quality control data. Help Center Articles Additional details about viral vector production at Addgene...various aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies...

  9. Optogenetics Plasmid Collection

    Type
    Collection
    ...Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial...the cell. When using these opsins as a tool to control neuronal activity, scientists generally attach ...localization. Many other types of proteins can also be controlled by light, generally by utilizing a light sensitive...fusions can then be used as an optical switch to control protein interactions, dimerization, localization...the table below for plasmids that use light to control a protein or cellular activity. For example, search...

  10. AAV for Neuronal Tracing

    Type
    Collection
    ...viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies...be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute...pAAV-TREtight-mTagBFP2-B19G AAV1 Ian Wickersham Introduction to Rabies Virus-based Monosynaptic Tracing The...-deleted rabies to specific starter cells, the tropism of this virus was modified so that it could only..., Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a deletion-mutant rabies...

  11. CRISPR References and Information

    Type
    Collection
    ...wide range of organims. Developed by the Michael Boutros Lab . CasFinder: Flexible algorithm for identifying...107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized...Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP)... packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for...

  12. Optogenetics AAV Preps

    Type
    Collection
    ... Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics...Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...

  13. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... store a record of invading phages but also to destroy the phages upon re-exposure. More specifically,...complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview...could be programmed for targeted DNA cleavage in vitro. In 2013, Cong et al. and Mali et al. described ...therapeutic applications. Despite the ethical controversies surrounding non-research applications, it’s ...Weissman JS. 2014. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell . 159(3...

  14. Recombinases AAV Preps

    Type
    Collection
    ...* Karl Deisseroth *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...

  15. Microbiology Resources

    Type
    Collection
    ... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...Methanococcus maripaludis Methanothermobacter thermautotrophicus Sulfolobus islandicus Plasmids for Bacteria...Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference...

  16. Chemogenetics AAV Preps

    Type
    Collection
    ...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t ...

  17. AAV Molecular Tools

    Type
    Collection
    ... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...Doxycycline-inducible expression of the reverse tetracycline-controlled transactivator (rtTA). 1 Wei Xu 183708 pAAV-CamkII-tTA...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for...mCherry-tagged, plasma membrane-tethered ibARK in astrocytes. 5 Jun Nagai Anterograde Transsynaptic Tracers...

  18. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo...chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo delivery where the vector is ...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...Francois St-Pierre 26737 GFP-UtrCH Actin filaments Utrophin calponin homology domain EGFP William Bement 26740... 26740 mCherry-UtrCH Actin filaments Utrophin calponin homology domain mCherry William Bement 164459 LifeAct-mGreenLantern...212020 pcDNA3/mStayGold(c4)=UtrCH Actin filaments Utrophin calponin homology domain mStayGold Atsushi Miyawaki...

  20. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al., 2018). This phosphosite library... (Plasmid #68307) ) at the central position. Introducing Hi-P for screening phosphorylation-dependent ...coli . The phosphosite DNA library can also be introduced into a second expression vector encoding a split...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...

  21. Zhang Lab CRISPR Page

    Type
    Collection
    ...recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase...within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone...targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is... L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33...

  22. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library...Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1)...reading frames with or without GFP and mCherry controls. Do you have suggestions for other plasmids that...

  23. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Parasite Resources Fully functional Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use DNA ... tables below for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other...

  24. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...find current pricing information Standard Quality Control Plasmid verified by next generation sequencing ...Plasmid Description Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...

  25. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...blocks the progression of the RNA polymerase, thus strongly decreasing the expression level. Mismatches between...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for...

  26. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ...

  27. Adenovirus Plasmids

    Type
    Collection
    ... packaging capacity, high efficiency in driving strong transient gene expression in both dividing and ...pShuttle-CMV-F2A-T2A-Venus For production of viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP...-CMV-F2A-T2A For production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids...

  28. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials are available...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...

  29. mTOR Pathway

    Type
    Collection
    ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...

  30. Worm Expression Resources

    Type
    Collection
    ...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...
Showing: 31 - 60 of 91 results