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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 68 results for: ANG

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Showing: 21 - 40 of 68 results

  1. Promega Plasmid Collection

    Type
    Collection
    ...specialized Promega technologies that support a range of applications. The technology includes NanoLuc...enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are ideal for... a fusion protein in your hands, thanks to interchangeable ligands. HaloTags form a covalent bond with...options. HiBiT technology's exceptional dynamic range can detect low-abundance proteins at endogenous ...

  2. TALEN Guide

    Type
    Collection
    ...targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove.... A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...available from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature...

  3. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual ...

  4. Mammalian RNAi Tools

    Type
    Collection
    ...Link opens in a new window) . Zhang, J., Chen, B., Gan, C., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive...in a new window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin RNA ...

  5. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from the Michael Bassik lab , in...

  6. Adenovirus Plasmids

    Type
    Collection
    ... (Addgene #16403) plasmid with Gateway cassette Zhang 50958 ShuttleNSE For production of viruses containing...viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP For production of viruses containing...production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids Browse commonly...

  7. Antibody Production

    Type
    Collection
    ...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody preps ... The PCR product is purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal adhesions Paxillin PSmOrange Vladislav Verkhusha 36991 Desmoglein2...pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-H2A Nucleus H2A mTurquoise2 Dorus...Clathrin-coated vesicles Clathrin light chain EYFP Xiaowei Zhuang 164462 Clathrin-mGreenLantern Clathrin-coated vesicles...

  9. Microbiology Resources

    Type
    Collection
    ...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

  10. Lentiviral Prep Service

    Type
    Collection
    ...resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating Gene Expression...none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) ...marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases... of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements... memory loss, deficits in decision-making, and language abilities. The disease can be classified as early-onset...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector, expressing...reduced background expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org...transcriptional activators: novel mutations yield expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (...

  13. KLF Research Plasmids

    Type
    Collection
    ...Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman Konrad Hochedlinger...Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family...

  14. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael Davidson...Yellow Plasmid Description Industry Return to top Orange Plasmid Description Industry Return to top Red ...

  15. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously...

  16. Resolute Plasmid Collection

    Type
    Collection
    ...properties, protein thermal stabilization, and changes of voltage all contribute to assess ligand action...allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid ...

  17. Recombinases AAV Preps

    Type
    Collection
    ... AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, ...pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Li Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Connie Cepko...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... flap is excised, the target sequence remains unchanged and available as a substrate for another round...BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3 pegRNA BsaI + HindIII No...

  19. Arf GTPase Family

    Type
    Collection
    ...regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase activating...help of Richard Kahn . References Klinger, C. M., Spang, A., Dacks, J. B., & Ettema, T. J. (2016). Tracing...

  20. Lentivirus Plasmids

    Type
    Collection
    ...Gateway plasmid for CMV driven expression of cDNA. See Yang et al. (2011) for additional variants. David Root...pooled screens. Part of the MORF Collection . Feng Zhang Return to top Popular Lentiviral Packaging Plasmids...
Showing: 21 - 40 of 68 results