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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36 results for: Arch-GFP

Showing: 21 - 36 of 36 results

  1. All Antibodies

    Type
    Collection
    ...like Myc, GFP, and more. Neuroscience : Antibodies targeting proteins for neuroscience research. Institute...Addgene values transparency and accessibility in research. For each antibody, we provide important details...their use, visit our Antibody Guide . Browse or search the table below or visit the following pages to...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... pX459 (Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively...CRISPR/Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells with 250...filter into a FACS tube. FACS sort the top ~3% of GFP positive cells in order to enrich for cells that ...perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica Scandinavica...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6-gRNA 79145...Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA...cut Lb Cpf1 Neo Welker AIO-GFP 74119 Mammalian U6x2 yes, nick S. pyogenes EGFP Jackson AIO-mCherry 74120...pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO.1-puro...pyogenes Zhang pLKO5.sgRNA.EFS.GFP 57822 Mammalian/Lentiviral BsmBI none S. pyogenes EGFP Ebert pSECC 60820...tagRFP657 Ebert pL-CRISPR.SFFV.GFP 57827 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO5.sgRNA.EFS.tRFP...Other/Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha...

  4. CRISPR References and Information

    Type
    Collection
    ...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...Lab . Cas-Designer (Link opens in a new window) Searches for targets that maximize knockout efficiency ...Signaling and Functional Genomics at the German Cancer Research Center (DKFZ) in Heidelberg. CRISPR FAQ forum ...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

  6. Worm Expression Resources

    Type
    Collection
    ...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...synthetic biology collections) for use in C. elegan research. Plasmid...been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...computer scientists dedicated to providing the research community with accurate, current, accessible information...

  7. Biosensor AAV Preps

    Type
    Collection
    ...-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors iATPSnFR2 5-HT...

  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...system of choice, promoter, or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen...been limited by low integration efficiency but research groups continue to optimize these tools. The best...recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort buttons to find plasmids based on ...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Flp recombinase...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Dre recombinase... gene expression may be activated or repressed. Search by recombinase (Cre, Flp, Dre, etc.) or target ...

  9. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20 Mitochondria...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin... SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin...101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin...109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43 Gap junctions 109121 HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone...

  10. The Pleiades Promoter Project

    Type
    Collection
    ...pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44...pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS ...pEMS1167 EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS ...pEMS1160 EGFP/NLS Ple93 GPR88 pEMS1161 EGFP/NLS Ple94 GPR88 pEMS1162 EGFP/NLS Ple95 GPR88 pEMS1163 EGFP/NLS...pEMS1216 EGFP/NLS Ple99 GRP pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102...pEMS1171 EGFP/NLS Ple151 OLIG1 pEMS1172 EGFP/NLS Ple152 OXT pEMS1237 EGFP/NLS Ple153 OXT pEMS1238 EGFP/NLS ...pEMS1262 EGFP/NLS Ple217 TAC1 pEMS1263 EGFP/NLS Ple218 TAC3 pEMS1181 EGFP/NLS Ple219 TAC3 pEMS1182 EGFP/NLS...

  11. Arf GTPase Family

    Type
    Collection
    ...EFA6) 5662 1024 Mammalian (pEGFP-C1) GEF Psd2 (EFA6C) 84249 771 Mammalian (pEGFP-C1), Gateway GEF Psd3 (EFA6D...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... RAS Clone Collections Richard...GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway GTPase Arl2 402 184 Bacterial (pET),...116984 1704 GAP Arap3 (CENTD3) 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 (CENTB1) 9744 740 Mammalian (pFLAG-CMV2...Bacterial (pET) GAP Git1 28964 770 Mammalian (pcDNA3, pEGFP) GAP Git2 (PKL, CAT2) 9815 759 GAP ELMOD1 55531 ...GAP RP2 6102 350 Bacterial (pGEX4T), Mammalian (pEGFP-N3), Gateway GEF Arfgef1 (BIG1, ARFGEP1) 10565 1849...398 Gateway GEF Cyth2 (ARNO) 9266 400 Mammalian (pEGFP-C1), Gateway GEF Cyth3 (ARNO3, GRP1) 9265 399 Gateway...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector...viral entry and infection. This approach allows researchers to safely study mechanisms of infection and to...coronaviruses using appropriate packaging plasmids. Use the search bar to find a particular viral spike protein (MERS...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...

  13. COVID-19 Resources

    Type
    Collection
    ...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...libraries . FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based ...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...

  14. CRISPR Guide

    Type
    Collection
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...promotes and advocates for zebrafish research in the international research community. The Latin American Zebrafish...based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease genes...model system for human disease and translational research, including the study of cancer, inflammation and...plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain ...plasmids that contain tools for use in zebrafish research. This includes plasmids for genome editing, empty...-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit ... and materials and information about zebrafish research. China Zebrafish Resource Center (CZRC) - A resource...

  16. TALEN Engineering

    Type
    Collection
    ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three... new version of ZiFiT Targeter enables users to search their sequences of interest for potential TALEN...TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with...
Showing: 21 - 36 of 36 results