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Showing: 21 - 37 of 37 results

  1. All Antibodies

    Type
    Collection
    ...like Myc, GFP, and more. Neuroscience : Antibodies targeting proteins for neuroscience research. Institute...Addgene values transparency and accessibility in research. For each antibody, we provide important details...their use, visit our Antibody Guide . Browse or search the table below or visit the following pages to...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... pX459 (Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively...CRISPR/Cas9 construct (10 μg total). Add 0.5 μg of GFP expression construct. Electroporate cells with 250...filter into a FACS tube. FACS sort the top ~3% of GFP positive cells in order to enrich for cells that ...perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica Scandinavica...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...phGluc Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher...Firefly TGB AAV expression of firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian...need a luciferase reporter for? Browse or use the search box to find a construct that contains your regulatory...

  4. CRISPR References and Information

    Type
    Collection
    ...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...tomato, corn, monkey (macaca mulatta). Cas-Designer searches for targets that maximize knockout efficiency ...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6-gRNA 79145...Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA...cut Lb Cpf1 Neo Welker AIO-GFP 74119 Mammalian U6x2 yes, nick S. pyogenes EGFP Jackson AIO-mCherry 74120...pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO.1-puro...pyogenes Zhang pLKO5.sgRNA.EFS.GFP 57822 Mammalian/Lentiviral BsmBI none S. pyogenes EGFP Ebert pSECC 60820...tagRFP657 Ebert pL-CRISPR.SFFV.GFP 57827 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO5.sgRNA.EFS.tRFP...Other/Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha...

  6. Plan Your Experiment

    Type
    Collection
    ...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...Started CRISPR is a powerful system that enables researchers to manipulate the genome like never before. This...framework to get you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

  8. Worm Expression Resources

    Type
    Collection
    ...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...synthetic biology collections) for use in C. elegan research. Plasmid...been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...computer scientists dedicated to providing the research community with accurate, current, accessible information...

  9. Biosensor AAV Preps

    Type
    Collection
    ...-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Schreiter Dopamine Sensors...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ATP Sensors iATPSnFR2 5-HT Sensors...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20 Mitochondria...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin... SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin...101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin...109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43 Gap junctions 109121 HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone...

  11. The Pleiades Promoter Project

    Type
    Collection
    ... pEMS1301 cre/EGFP/NLS N/A pEMS1308 EGFP/cre N/A pEMS1302 EGFP/cre/NLS N/A pEMS1306 EGFP/NLS N/A pEMS1307...pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44...pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS ...pEMS1167 EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS ...pEMS1160 EGFP/NLS Ple93 GPR88 pEMS1161 EGFP/NLS Ple94 GPR88 pEMS1162 EGFP/NLS Ple95 GPR88 pEMS1163 EGFP/NLS...pEMS1216 EGFP/NLS Ple99 GRP pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102...pEMS1171 EGFP/NLS Ple151 OLIG1 pEMS1172 EGFP/NLS Ple152 OXT pEMS1237 EGFP/NLS Ple153 OXT pEMS1238 EGFP/NLS ...

  12. Arf GTPase Family

    Type
    Collection
    ...GEF Psd 5662 1024 Mammalian (pEGFP-C1) GEF Psd2 84249 771 Mammalian (pEGFP-C1), Gateway GEF Psd3 23362 ...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... Ras Pathway RAS Clone Collections...GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway GTPase Arl2 402 184 Bacterial (pET),...Arap2 116984 1704 GAP Arap3 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 9744 740 Mammalian (pFLAG-CMV2) GAP...Bacterial (pET) GAP Git1 28964 770 Mammalian (pcDNA3, pEGFP) GAP Git2 9815 759 GAP ELMOD1 55531 334 Bacterial... 9267 398 Gateway GEF Cyth2 9266 400 Mammalian (pEGFP-C1), Gateway GEF Cyth3 9265 399 Gateway GEF Cyth4...23362 1047 Gateway GEF Psd4 23550 1056 Mammalian (pEGFP-C1) GEF Fbxo8 26269 319 Gateway Return to top The ...

  13. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector...viral entry and infection. This approach allows researchers to safely study mechanisms of infection and to...coronaviruses using appropriate packaging plasmids. Use the search bar to find a particular viral spike protein (MERS...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...

  14. COVID-19 Resources

    Type
    Collection
    ...protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols : Protocols...libraries . FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based ...can be found here: ASM COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI...plasmids and resources available for Coronavirus research. Collections...and Coronavirus Plasmids & Resources The global research community is moving quickly to expand the knowledge...available that may be useful for COVID-19 related research. Additionally, we have linked to collections of...Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17...

  15. CRISPR Guide

    Type
    Collection
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...promotes and advocates for zebrafish research in the international research community. The Latin American Zebrafish...based gene editing make the zebrafish a stalwart research model. With more than 70% of human disease genes...model system for human disease and translational research, including the study of cancer, inflammation and...plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain ...plasmids that contain tools for use in zebrafish research. This includes plasmids for genome editing, empty...-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit ... and materials and information about zebrafish research. China Zebrafish Resource Center (CZRC) - A resource...

  17. TALEN Engineering

    Type
    Collection
    ...assemble TAL effector repeat arrays on a single architectural framework and the DNAs produced by all three... new version of ZiFiT Targeter enables users to search their sequences of interest for potential TALEN...TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with...
Showing: 21 - 37 of 37 results