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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 82 results for: IGH@;

Showing: 21 - 40 of 82 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF,...95% of archaeal and 48% of bacterial genomes, are highly diverse, with varying PAM sequences and number/...) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive...creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The first... Genome engineers continue to work to develop a highly specific, programmable platform well-suited for...MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable...

  2. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread in the...Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction of the mouse retina by tyrosine-mutant...a mouse model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39....acknowledge Arun Srivastava and cite Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...

  3. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre , other recombinases... iCre or FLPo in mammalian cells may facilitate higher rates of recombination. Gene Expression Strategies... leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below to find popular...search for "split", "rapamycin", “tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description...

  4. E11 Bio PRISM Collection

    Type
    Collection
    ...Learn about PRISM, a set of molecular tools for high-throughput reconstruction of individual neurons ...is a set of methods and molecular tools enabling high-throughput reconstruction of individual neurons ...expansion microscopy, allowing for super-resolution light microscopy. In brain tissue, these protein barcodes...used in conjunction with Cre-enhancer viruses for highly focused circuit mapping and reconstruction. Potential...used in expansion microscopy workflows to achieve high-resolution optical imaging down to individual synapses...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ...deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Ian Wickersham Introduction...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

  6. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Adenoviral vectors have high packaging capacity, high transduction efficiency, and high but transient transgene...to as just retroviral vectors, have a relatively high transduction efficiency and only infect dividing...linear DNA 5–36 kb 105 nm Dividing/Non-dividing No High *Recombinant AAV has a low frequency of target cell...

  7. Institute for Protein Innovation

    Type
    Collection
    ...combination allows IPI to generate antibodies to highly conserved proteins and other difficult-to-target...part of that mission, IPI is providing access to high-quality, well-characterized antibodies in an open...IPI’s Synaptic Cleft Antibody Collection features highly selective recombinant monoclonal antibodies targeting...Return to top Glypicans Glypicans are a family of highly conserved proteoglycans crucial for regulating ...

  8. Distribution to Industry

    Type
    Collection
    ...Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly.... biological materials and intellectual property rights, and provides liability protection for material...qualified for-profit entities. Addgene reserves the right to determine if an entity is qualified to receive...

  9. Worm Expression Resources

    Type
    Collection
    ...types and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene...Plasmid Tools for Use in Worms The table below highlights plasmids that contain tools for use in worm research...

  10. Viral Production

    Type
    Collection
    ...optimized for each specific virus in order to generate high quality preparations. After production, all virus...titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method...digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing...staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...neuroscience -related antibodies. Find antibodies to highly conserved proteins and other difficult-to-target...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

  12. Rett Syndrome

    Type
    Collection
    ...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

  13. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ...

  14. AAV Viral Preps

    Type
    Collection
    ...the lowest biosafety protection levels. High quality and high titer in-stock AAV preps are affordable,... density centrifugation. Addgene’s comprehensive high-quality quality control process includes titering...

  15. Chemogenetics Plasmids

    Type
    Collection
    ...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

  16. Recombinases AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of... Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter Fluorophore...

  17. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

  18. Antibody Production

    Type
    Collection
    ... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

  19. Retrovirus Plasmids

    Type
    Collection
    ...— contains packaging gene env This collection highlights popular gamma-retroviral transfer, packaging,...Foijer 47916 pRXTN CMV/MSV A modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart...

  20. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Core) and Addgene previously partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...
Showing: 21 - 40 of 82 results