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...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding of CRISPR ...genomic target of the gRNA can be any ~20 nucleotide sequence, provided it meets two conditions: The sequence...fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers have...Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) ...

... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...

...control gRNAs . All-in-one Plasmids (gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker PI...PI Publication Return to top gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI Publication...Publication Return to top Multiplex gRNA Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI... by visiting our CRISPR reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols gRNA Design Tools...

...Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid Gene/Insert ...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Genomic Loci Mammalian Bacteria Yeast Resources Identifying molecules associated with a genomic region of... are more straightforward and enable direct identification of molecules associated with a genomic region... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...thousands of fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent...animals. Additional Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit...control molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to ...

...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...

...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid...Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...

...viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . Resources Addgene’s ...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...find viral production and transfer plasmids for a wide array of research purposes. Interested in Addgene's...permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors...

...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Return to top Other Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter..., Dre, etc.) or target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene...selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...

...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent...these membrane trafficking pathways and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...Labeling these structures can be quite useful for identifying the localization or possible function of a protein...

...product of exctinction coefficient and quantum yield divided by 1,000. Note that the effective brightness of...

... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...

...Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...backbone plasmids for adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral...bacterial strains for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain...Adenoviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 YouTube Videos Addgene's ...Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery due to their high packaging...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...adenoviral vector production. Read our adenoviral vector guide for more information about adenoviral components...

...availability, viral packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging... produce AAV vectors for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...this table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert PI Return to top AAV Helper Plasmids...in Addgene's repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources...AAV) Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's ...offer precise delivery of genetic material across a wide range of tissue types with minimal immune response...required for AAV viral replication. This collection provides a curated selection of transfer, packaging, and...

...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...

..."worm". Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...