Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 92 results for: Vars;

Showing: 21 - 40 of 92 results

  1. TALEN Guide

    Type
    Collection
    ... a large number of zinc fingers that recognize various nucleotide triplets. With some trial and error,...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...31 repeats can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). ...

  2. Validated gRNA Sequences

    Type
    Collection
    ...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...gRNA activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with...elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...

  3. Viral Vectors

    Type
    Collection
    ... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses... immunogenicity. Different types of viruses also vary in the composition of their viral genome, with some...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...

  5. Rett Syndrome

    Type
    Collection
    ...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol...

  6. Optogenetics AAV Preps

    Type
    Collection
    ...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws WiChR stGtACR iC++ OPN3 PdCO PPO...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...enzyme responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic ...the protein is available, making it ideal for a variety of applications. To learn more and plan your experiment...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate...

  8. Bacterial Expression Systems

    Type
    Collection
    ...whose expression levels can be controlled by a variety of small molecules, light, and temperature, in ...#1000000037) contains specific bacterial promoters that vary in strength and that can be used to reliably drive...constructs. The Zinc Finger Consortium page contains a variety of reagents for engineering and expressing zinc...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...expression, the system has been engineered with a variety of improvements. A synthetic Tet Response Element...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber...

  10. Biosensor AAV Preps

    Type
    Collection
    ...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should...proteins. General Structures The cell contains a variety of organelles and subcellular structures each with...

  13. Brain Initiative Collection

    Type
    Collection
    ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...

  14. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

  16. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

  17. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

  18. CRISPR Plasmids and Resources

    Type
    Collection
    ...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

  19. Retrovirus Plasmids

    Type
    Collection
    ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

  20. Antibody Production

    Type
    Collection
    ...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...
Showing: 21 - 40 of 92 results