We narrowed to 90 results for: Vars;
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TypeCollection...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...
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Rett Syndrome
TypeCollection...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol... -
Optogenetics AAV Preps
TypeCollection...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV... -
Luciferase Plasmid Collection
TypeCollection...enzyme responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic ...the protein is available, making it ideal for a variety of applications. To learn more and plan your experiment...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate... -
Bacterial Expression Systems
TypeCollection...whose expression levels can be controlled by a variety of small molecules, light, and temperature, in ...#1000000037) contains specific bacterial promoters that vary in strength and that can be used to reliably drive...constructs. The Zinc Finger Consortium page contains a variety of reagents for engineering and expressing zinc... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Enhancers were evaluated in mouse brains, using various delivery methods including intracerebroventricular...contains best-in-class AAV plasmids to target a variety of cell populations in different brain regions...., G., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird,... -
Tetracycline Inducible Expression
TypeCollection...expression, the system has been engineered with a variety of improvements. A synthetic Tet Response Element...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. To compare... General Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each ... -
Biosensor AAV Preps
TypeCollection...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell... -
Brain Initiative Collection
TypeCollection...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ... -
Brzezinski Lab CRISPR Collection
TypeCollection... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting... -
Botman-Teusink Yeast FP Collection
TypeCollection...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression... -
CRISPR Plasmids and Resources
TypeCollection...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific... -
Retrovirus Plasmids
TypeCollection...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory... -
Antibody Production
TypeCollection...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced... -
mTOR Pathway
TypeCollection...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream... -
Promega Plasmid Collection
TypeCollection...which have been developed for special functions that vary from purification, visualization, and functional...specific purpose. HaloTag technology is optimized for various protein expression systems including E. coli , ...