...of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252/msb.20177899. PMID: 29519933...attachment site of the phage 186. Detailed information about the repression system can be found in this article...29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this...

...). DeChenne-Peters et al. PLoS One. 2023 Mar 9;18(3):e0282170. PMID: 36893201 (Link opens in a new window...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

...Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

... consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...

...cutting enzymes: NotI at the 5' end and AscI at the 3' end. These inserts can then be sub-cloned into a ...important in cellular recognition processes. Laboratories interested in these approaches have created ... and secreted proteins Mouse sperm Mouse egg Laboratories using AVEXIS: Christian Sollner, Max Planck ...

... work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse Available PHP.eB...Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory...systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in ...AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type...

...Combinatorial Strategies Connie Cepko 2:45 PM Break 3:00 PM (Group Discussion) Technologies and Resources...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

... rpoZ- on plates lacking histidine and containing 3-AT Scot Wolfe Bacterial one-hybrid system for testing...promotes growth on plates lacking His and containing 3-AT. FLIParaF.Ec-250n 18855 N/A FRET between CFP and...in vitro ? These, in addition to many questions about bacteria themselves, can be answered using model...overexpressing or knocking down a gene can tell you much about its function and/or alter cellular activity in a...basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic compound, there...once you get it right, you can learn many things about your protein’s function, structure, and interactions...molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often times, ...

...genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab maintenance. ... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...

...Cre lac Bacterial Ekker 47551 pDD104 (Peft-3::Cre) Cre eft-3 C. elegans Goldstein 48201 CAG-GFP-IRES-CRE...deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft-3 C. elegans Jorgensen 75267 pAAV-CIBN-CreC photo-activatable...Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104. Epub 2007 ...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...

...Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

...TALEs for the masses. Rusk N. Nat Methods 2011 Mar;8(3):197. Pubmed PMID: 21473016 . Move over ZFNs. Nat ...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

...standard consisting of ECFP and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

...shRNA cassettes into the unique SnaBI site in the 3´-LTR). See here for other versions of pULTRA. Moore... Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...systems Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral... or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono...

... and breathing, and often results in death within 3-5 years from symptom onset. Disease mechanisms are...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

...contains a potential cleavage site for furin proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

...: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window) PMID: 16615965... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

...Optogenetics Boyden 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics Fishell 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

...1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Fishell 135634...and Optogenetics Guide page for more information about this field. Use the table and filters below to find...

...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...