...Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB...following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann... Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has produced...tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use... from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly... CRISPR tagging technique for use in Drosophila cells that uses PCR to generate both an expression cassette...

...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific flanking...proteins can be used to image genomic loci in live cells. RNA targeting : Cas13 enzymes target RNA rather...non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used ...

...systems were tested for functionality in mammalian cells. The lac system with the inducer isopropylthio-β-galactoside... and Bujard tested the tet system in a mammalian cell system (HeLa) and found that the tet system was ... interest rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system...derivatives can be viewed in Krueger et al, 2004. Some cell culture sera may contain tetracycline or its derivatives...of gene expression at a single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under...vector lentiviral Gateway RNAi system for conditional cell line generation; contains expression cassette for...CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance...

...protein-protein interactions and nerve cell function within the cell. Parkinson's Disease Parkinson’s disease...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of ...that causes the progressive degeneration of nerve cells in the brain. Symptoms include changes in behavior...FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere et al. Mol Neurodegener. 2023 Aug 8...system, peripheral nervous system, vesicular brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40...and cortical neuron differentiation. Held et al. Cell Rep. 2023 Aug 30. Enhance differentiation to mesencephalic...neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based method to monitor ...

...molecular switches that transmit signals within the cell. GTPases have an active (GTP) and inactive (GDP)...prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and differentiation...Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate a variety of ...pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential...essential for regulating the growth of the cell, however when mutations occur in the Ras gene it can be... active form, constantly activating pathways for cell growth and potentially leading to cancer. Constitutively...E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor...

...between animal cells. They are important for holding cell structures together and reducing cell stress. There...give the cell its shape, organize cellular organelles, and are necessary for movement and cell division...example, houses the cell’s genetic material. The mitochondria produces the energy cells required for chemical... on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle...Fluorescent Protein Collection . Return to top Cell Junctions Cell junctions are responsible for forming connections... General Structures The cell contains a variety of organelles and subcellular structures each with their...its role in secreting proteins from the cell to the extracellular environment. Proteins are first translated...

...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting... multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... for detecting the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for... loci, as well as chromosome painting in living cells. Visualizing a specific genomic locus requires recruitment...repetitive loci can be efficiently visualized in living cells using a single gRNA that has multiple targeted sequences...

...adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral...serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning. The AAV2(trpYF...displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V) serotype was ...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents and primates...human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3...delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of... of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation...

...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids... one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet...

...reverse transcriptase and integrase. Once in a target cell, the virus uses the reverse transcriptase in tow...γ-retroviruses are that they can only transduce dividing cells (this is because they are only able to enter the...of Large T antigen for creation of immortalized cells (see Weinberg Lab page for resistance variants) ...Expression of hTERT for creation of immortalized cells; additional plasmids include Puro resistance and...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified version...MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions for other...

...Scanziani Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression...Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators and Inducible...diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3...expression of designer pro-taCasp3 and TEVp for studying cell ablation. 5 Shah , Wells Don’t See What You’re Looking...

...function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008 , Foley & Yeh et al., PLoS ONE... Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources A detailed protocol for practicing...ZFNs that function in zebrafish, plants, and human cells Kits...

...to target a variety of cell populations in different brain regions. Labeled cell populations were tested...regulatory sequences — termed enhancers — to drive cell type-specific expression. The Allen Institute for...differential chromatin accessibility across different cell types and cloned into an AAV backbone. Most enhancers...mouse brains. This collection contains plasmids for cell populations in the following regions: Isocortex ...-WPRE3-BGHpA AiP12320 AiE0475m SYFP2 Chandelier cells (Pvalb) Isocortex 230384* pAAV-AiE0475m-minBG-iCre...pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA AiP13790 AiE0475m Cre Chandelier cells (Pvalb) Isocortex 214561 pAAV-AiE2566m-minBG-SYFP2...pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2...

...SARS-CoV-2 . TMPRSS2 - Hoffmann M, et al, 2020, Cell SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is...key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...Human Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV...2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor ...2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease...increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia... Combat SARS-CoV-2 and Influenza. Abbott, et al. Cell. 2020 May 14;181(4):865-876.e12. (Link opens in ...

...Consortium plasmids that encode the extracellular regions of cell surface receptor and secreted proteins...expression plasmids that encode the extracellular regions of cell surface receptor and secreted proteins...and Wright GJ Molecular & cellular proteomics 2010 Dec; 9(12):2654-65. A cell surface interaction network...The inserts that encode the whole ectodomains of cell surface receptors are flanked by two 8 bp cutting...microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm Mouse ...screens or cellular assays to identify novel interactions or pathways that are important in cellular recognition...AVEXIS (for Avidity-based extracellular interaction screen) in which the extracellular regions of receptor ...

...transiently transfected cells. Co-expression of both plasmids in HEK293 cells could cause up to 2~3-fold...Weissman JS, Arkin AP, Lim WA. Cell . 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. PubMed PMID...JA, Lim WA, Weissman JS, Qi LS. Cell . 2013 Jul 9. doi: 10.1016/j.cell.2013.06.044. PubMed PMID 23849981...expression of arbitrary genes in bacteria or human cells ( Qi et al. ). This CRISPR interfering system (CRISPRi...optimized dcas9 gene under the control of Murine Stem Cell retroVirus LTR promoter. The second plasmid ( pgRNA_humanized...(CRISPRi), works independently of host cellular machineries, requiring only a nuclease-deficient Cas9 ...

... optical switch tools to measure and manipulate cells and their biomolecular processes....to blue light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered...improve the expression and membrane targeting in host cells. Other microbial opsins, such as the Halorhodopsins...actively move cations or anions into or out of the cell. When using these opsins as a tool to control neuronal...plasmids that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription...

...barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral...

...then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased...tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats...tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1...including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under... above helps you choose the best capsid for your cell type. First, do you want to target CNS ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or...AAV-PHP.eB with GRE or transgenic animal Brain vascular cells - AAV9-X1.1, AAV1-X1 Neurons - AAV.CAP-B10, AAV-...

...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the.... This buffer is not compatible for use in live cells and will interfere with conjugation. For applications...antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the antibody and a species-specific...Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane...