We narrowed to 89 results for: cha;

...Tet-regulated expression systems. Left: natural TetR mechanism; center: Tet-Off; right: Tet-On. TRE: Tet response...#21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid...expresses tTA from the CAG promoter. See article ( Chan et al., 2017 ) for other viral tTA plasmids. tTA...Neurodegenerative Disease Initiative (iNDI) Collection . Michael Ward 105840 pUCM-AAVS1-TO-hNGN2 Introduction of...for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA Helper...Rheenen 104102–104112 TetBow Series of plasmids for stochastic multicolor labeling for discriminating between...7792603 (Link opens in a new window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R...

...dependent 5, 9 Michael Bruchas 139505 pAAV-Ef1a-DIO-PPO-Venus EF1a PPO Venus Cre dependent 8, 9 Michael Bruchas...our optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more. Viral... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...

...nlsdTomato Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell...BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the AAV decision...Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed 28671695 (Link opens in a new...

...Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is...mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53... the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic...

... mutations have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is...transcriptional targets is ever-expanding; the best-characterized effects of p53 are in promoting cell cycle arrest...Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour suppression. Bieging KT...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

...injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9(E531D) The AAV44.9...Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular... of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID... AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;...

...to measure protein interactions or conformation changes....to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other ...intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and acceptor are...pairs with mTurquoise2 Mastop et al. (2017) Characterization of a spectrally diverse set of fluorescent...proteins, positive controls for many FRET pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live...

...Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of ... on protein-protein interactions, drug-induced changes, and more. NanoBit (Link opens in a new window)...

...tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection can ... Goedhart, J., Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:...

...mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from the Michael Bassik lab , in which Cas9 is fused...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...

...Request Service is subject to Addgene’s Terms of Purchase and the applicable material transfer agreements...knowledge from the start. However, we know there’s a chance that things may not turn out as expected. In the...materials. Please be aware that additional customs charges may be required before your order will be delivered...courier will contact you directly regarding these charges, if applicable....

... J.Y., Chan, S.W., Damstra, H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M.,...brain tissue. PRISM begins with the delivery of stochastic combinations of cell-filling antigenically distinct...individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled AAVs delivered in vivo generates...

...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...

...types of edits, including point mutations, base changes, and large insertions or deletions. For more information...how to validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...protein domain. Targeting a 5’ exon reduces the chances that the targeted region is removed from the mRNA...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...

...plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable...plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084 pLV-mCherry 3rd CMV driven expression ...pHAGE-mt-mKeima 3rd Expression of mitochondrial mKeima. Richard Youle 119816 pLentipuro3/TO/V5-GW/EGFP-Firefly ...integrase-deficient lentivirus. David Rawlings and Andrew Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases...

... CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC) and is brought to the ribosome where...incorporates phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein... The Rinehart and Söll labs at Yale University changed the way researchers can explore important questions...improved phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing...

...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to red over time... Resources Addgene Collections Empty Backbones Michael Davidson Fluorescent Protein Collection Luciferase...Fluorescent Protein Fusion Construct Kit Neveu Lab MXS Chaining Kit Lindquist Lab Yeast Gateway Kit Reading and...

... continue to optimize these tools. The best characterized SSR systems, Cre and Flp, are tyrosine recombinases... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

...interest by fusing it to a fluorescent protein. Michael Davidson Fluorescent Protein Collection Browse ...protein-protein interactions or conformational changes within a protein. Biosensors Monitor small biomolecules...organelles and subcellular structures with well-characterized marker proteins. In Vivo Imaging Image with ...

...can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR..., the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems are ...components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or ...