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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 98 results for: genome

Showing: 21 - 40 of 98 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ...specific modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco...variety of molecular biology technologies: from genome engineering and gene regulation to fluorescent ...biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes - Zhenghe...system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang... virus (TRV) that simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return ... agriculture and conservation. Medicinal Plant Genomics Resource (Link opens in a new window) - Transcriptomic...

  2. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1 promoter that...Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression of the tTA from the...Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized mRuby2 from the...

  3. Browse

    Type
    Collection
    ...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro...Fusion Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable...can also be used for for viral vector delivery, genome modification, reporter assays, shRNA expression... efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids...nondividing cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination...Retroviral Easy and safe to use, integrate into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian...efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for...

  5. Microbiology Resources

    Type
    Collection
    ...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

  6. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

  7. Viral Production

    Type
    Collection
    ...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

  8. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce...Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derived from fungi and bacteriophages are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

  10. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy in living cells...sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target locations can include...

  11. Lentivirus Plasmids

    Type
    Collection
    ...These viruses also integrate stably into the host genome, enabling long term transgene expression. There...because this virus integrates randomly into the host genome, it has the potential for insertional mutagensis...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This ...Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material enclosed in the viral...lentivirus is used for research, it is the lentiviral genome that encodes genetic material that the researcher... wants delivered to specific target cells. This genome is encoded by plasmids called "transfer plasmids...

  12. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins...

  13. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...

  15. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...

  16. Lentiviral Prep Service

    Type
    Collection
    ...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

  18. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  19. Bacterial Expression Systems

    Type
    Collection
    ...Experiments Return to top Genome Engineering Modifying the bacterial genome by knocking out genes or introducing... CRISPR-based Genome Editing Systems For Editing in E. coli INTEGRATE: Bacterial Genome Engineering Using...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic Code Expansion... Protein Purification Resources The Structural Genomics Consortium (SGC) collection page contains an extensive...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...

  20. Synthetic Biology - Overview

    Type
    Collection
    ...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J....
Showing: 21 - 40 of 98 results