Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 81 results for: genome

Clear keywords
Showing: 21 - 40 of 81 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro...Fusion Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable...can also be used for for viral vector delivery, genome modification, reporter assays, shRNA expression... efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids...nondividing cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination...Retroviral Easy and safe to use, integrate into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian...efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for...

  2. Microbiology Resources

    Type
    Collection
    ...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

  3. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

  4. Viral Production

    Type
    Collection
    ...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derive from fungi or bacteriophages and are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

  6. Lentiviral Prep Service

    Type
    Collection
    ...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...

  8. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  9. Bacterial Expression Systems

    Type
    Collection
    ...Experiments Return to top Genome Engineering Modifying the bacterial genome by knocking out genes or introducing... CRISPR-based Genome Editing Systems For Editing in E. coli INTEGRATE: Bacterial Genome Engineering Using...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic Code Expansion... Protein Purification Resources The Structural Genomics Consortium (SGC) collection page contains an extensive...Bacterial CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with...

  10. Synthetic Biology - Overview

    Type
    Collection
    ...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J....

  11. Adenovirus Plasmids

    Type
    Collection
    ...adenoviral plasmid containing the rest of the adenoviral genome necessary for viral production and homology regions...plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer Vogelstein 18703 pEZYshuttle Modified...that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction Bunz...

  12. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene...Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector. The PITCh donor plasmid...vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid...-programming sgRNA and HR donors for selectable genome modification. This system can be used to create...

  15. Zinc Finger Consortium Reagents

    Type
    Collection
    ... these reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish...expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents...

  16. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces...

  17. Retrovirus Plasmids

    Type
    Collection
    ...of retrovirus and contain a viral genome encoded on RNA. The genome includes a reverse transcriptase and... and an integrase to insert the viral genome into a host cell. Scientists can take advantage of this to...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ... Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering... Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like...target DNA sequences in living cells (TALE-mediated Genome Visualization [TGV]). pTALYM3 and pTALYM4 contain...this page are designed to target and edit the epigenome of putative enhancers in mammalian cells. These...target locus ( Mendenhall et al., 2013 ). This epigenome editing was also shown to alter regulation of ...

  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...

  20. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been...
Showing: 21 - 40 of 81 results