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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 43 results for: grna

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Showing: 21 - 40 of 43 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  2. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  6. Lentiviral Prep Service

    Type
    Collection
    ...an RNA guide (gRNA) that harbors targeting sequences. Browse our collection of empty gRNA plasmids . For...controls Puromycin John Doench , David Root 73179 Human gRNA library Brunello in lentiCRISPRv2 Human sgRNA library...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76361 TBK1 gRNA (BRDN0001145663) TBK1...David Root 76362 TBK1 gRNA (BRDN0001148392) TBK1 U6 ALS David Root 76363 TBK1 gRNA (BRDN0001148607) TBK1...David Root 76470 GAK gRNA (BRDN0001146687) GAK U6 Parkinson's David Root 76471 GAK gRNA (BRDN0001146263) ...David Root 76472 GAK gRNA (BRDN0001148048) GAK U6 Parkinson's David Root 76809 PANK2 gRNA (BRDN0001149459)...

  9. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  10. Lentivirus Plasmids

    Type
    Collection
    ...to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid...

  11. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  15. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...Plasmids and Resources collection, and browse our Empty gRNA Expression Vectors page Cre-lox Site-specific recombination...

  16. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  17. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from the Zhang lab...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...
Showing: 21 - 40 of 43 results