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We narrowed to 46 results for: grna

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Showing: 21 - 40 of 46 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  2. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  4. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  7. Lentiviral Prep Service

    Type
    Collection
    ...an RNA guide (gRNA) that harbors targeting sequences. Browse our collection of empty gRNA plasmids . For... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone...

  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76361 TBK1 gRNA (BRDN0001145663) TBK1...David Root 76362 TBK1 gRNA (BRDN0001148392) TBK1 U6 ALS David Root 76363 TBK1 gRNA (BRDN0001148607) TBK1...David Root 76470 GAK gRNA (BRDN0001146687) GAK U6 Parkinson's David Root 76471 GAK gRNA (BRDN0001146263) ...David Root 76472 GAK gRNA (BRDN0001148048) GAK U6 Parkinson's David Root 76809 PANK2 gRNA (BRDN0001149459)...

  10. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  11. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last ...

  13. Lentivirus Plasmids

    Type
    Collection
    ...to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors...Plasmids and Resources collection, and browse our Empty gRNA Expression Vectors page Cre-lox Site-specific recombination...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...Lentiviral expression plasmid for two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction...confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice...

  17. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  18. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...

  20. Zhang Lab CRISPR Page

    Type
    Collection
    ...CRISPR Cas9 gRNA plasmids from the Zhang lab...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...
Showing: 21 - 40 of 46 results