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Showing: 21 - 40 of 130 results
  1. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...
  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...
  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid Gene/Insert ...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
  4. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Genomic Loci Mammalian Bacteria Yeast Resources Identifying molecules associated with a genomic region of... are more straightforward and enable direct identification of molecules associated with a genomic region... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
  5. Caltech Systemic Capsids

    Type
    Collection
    ...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...
  6. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...contains thousands of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent...mammals. Additional Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit...control molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to ...
  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid...Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...
  9. Viral Vector Guides and Plasmids

    Type
    Collection
    ...viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . Resources Addgene’s ...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...find viral production and transfer plasmids for a wide array of research purposes. Interested in Addgene's...permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors...
  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Return to top Other Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter..., Dre, etc.) or target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene...selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...
  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent...these membrane trafficking pathways and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...Labeling these structures can be quite useful for identifying the localization or possible function of a protein...
  12. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...
  13. Adenovirus Plasmids

    Type
    Collection
    ...Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...backbone plasmids for adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral...bacterial strains for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain...Adenoviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 YouTube Videos Addgene's ...Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery due to their high packaging...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...adenoviral vector production. Read our adenoviral vector guide for more information about adenoviral components...
  14. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...availability, viral packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging... produce AAV vectors for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...this table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert PI Return to top AAV Helper Plasmids...in Addgene's repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources...AAV) Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's ...offer precise delivery of genetic material across a wide range of tissue types with minimal immune response...required for AAV viral replication. This collection provides a curated selection of transfer, packaging, and...
  15. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...
  16. CRISPR Plasmids - C. elegans

    Type
    Collection
    ..."worm". Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
  17. Retrovirus Plasmids

    Type
    Collection
    ...for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA...to top Popular Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV ...to top Popular Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended...Gamma-Retroviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource Guide Content last reviewed...envelope plasmids. Read our Gamma-retroviral Vector Guide for more information about gamma-retroviral components...production, and viral safety. Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want ...
  18. COVID-19 Resources

    Type
    Collection
    ...COVID-19 that may be of use to scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV...please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene...Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control...SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene...detection assays Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding... or Inserts Several mammalian genes have been identified as having a key role in coronavirus infection...and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools Browse the...
Showing: 21 - 40 of 130 results