...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...

...correct insert (gRNA) in pX330 / pX335 with BbsI/AgeI double digestion. As a successful insertion will destroy... have been published with thorough step-wise instructions on designing and using Cas9-based genome engineering...vector (like PX330). For large changes (>100bp insertions or deletions), we typically use plasmid donor...homology arms on each side flanking your desired insertion or mutation. Each arms is around 800bp. For transfection...CRISPR/Cas9 system to create specific mutations or insertions by Homologous Recombination (HR), what is the...purified long oligos. For large changes (>100bp insertions or deletions), the Zhang lab typically uses a...homology arms on each side flanking your desired insertion or mutation. Each arm is around 800bp. For transfection...

... EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains insulator sequence; plasmid...Blasticidin selection. Please NOTE, this vector contains an insert (MAT2A) which would need to be replaced by...Backbones Find a construct that will allow you to insert and induce your gene of interest. ID Plasmid Description...format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral...bidirectional tet-responsive promoter (Pbi); Pbi contains a TRE between two minimal CMV and is silent in...third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent in absence of binding...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria Plasmid Gene/Insert Promoter PI ... Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant Plasmid Gene/Insert Promoter Selectable... Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus Plasmid Gene/Insert Promoter Selectable...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Selectable Marker PI Publication Parasites Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

... Plasmid Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent...fluorescent. A variety of organic dyes and fluorescent proteins have been developed for use as donor and acceptor...intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor molecules...suitable for creating individual fluorescently tagged proteins to study protein-protein interactions and a series...laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET The following plasmids can be used to...brightness standard consisting of two Venus fluorescent proteins connected via a 5 amino acid linker V17V Anisotropy...brightness standard consisting of two Venus fluorescent proteins connected via a 17 amino acid linker V32V Anisotropy...

...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...Service: AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties ...transfer (FRET) efficiency of a pair of fluorescent proteins. Genetically encoded biosensors can be introduced...Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul...nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base... been designed for use in Drosophila and other insects. CRISPR... been designed for use in Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...a much lower efficiency than NHEJ. Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

...) that contains dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that contains sgRNA targeting...CRISPRi is a system that can regulate a genome instead of modifying a genome. 1. Two-plasmid CRISPRi system...knockdown The first plasmid ( pdCas9_bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene...resistance. The second plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ...knockdown The first plasmid ( pdCas9_humanized ) contains a human codon optimized dcas9 gene under the control...promoter. The second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette...sequences in the genome. The pgRNA_humanized also contains a CMV-puro-t2A-mCherry expression cassette, useful...

...reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible base-to-base...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...a much lower efficiency than NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically...on the opposite strand) mutations. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...via homology-directed repair (HDR). Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime...

...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...a much lower efficiency than NHEJ. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base ...on the opposite strand) mutations. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick ...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate...-activator to your specific locus. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...

...Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization More...as these well-characterized proteins. General Structures The cell contains a variety of organelles and...role in secreting proteins from the cell to the extracellular environment. Proteins are first translated...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors...has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular...FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...

...sgRNA without PBS. It contains extra sequences for BsaI digestion for insertion of PBS 71899 pAC1399-pX-sgRNA...These PUF-binding sites recruit PUF (Pumilio/FBF) domains that are fused to an effector, like those for epigenetic...alternate strategy to techniques that fuse effector domains to dCas9. PUF can be programmed to recognize virtually...loci. It can also potentially recruit different proteins on a single sgRNA allowing the study of protein...the future, modules tethering different fusion proteins can be assigned a unique PUF/PBS pairing. The ...4xNLS_PUFa_2xNLS NLSPUFa pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI)...4xNLS_PUFb_2xNLS NLSPUFb pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI)...

...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

...PubMed . Direct identification of insulator components by insertional chromatin immunoprecipitation. Fujita... Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation (iChIP) and engineered...with engineered DNA-binding molecules such as TAL proteins and the CRISPR system consisting of a catalytically...s13104-018-3486-3. PubMed . Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative ...enChIP technology using recombinant CRISPR ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. ...system using recombinant exogenous DNA-binding proteins.Fujita T, Fujii H. BMC Mol Biol. 2014 Nov 27;15:26.../s12867-014-0026-0. PubMed . Identification of proteins associated with an IFNgamma-responsive promoter...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...with concomitant expression of aggregation-prone proteins with the iPSC toolbox . Lam et al. bioRxiv. 2022...Foundation is dedicated to finding a cure for Parkinson's disease through an aggressively funded research...

...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...system to make singly or multiply phosphorylated proteins (Pirman et al., Nat Comm 2015). Improved phosphoprotein...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express ...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...phosphosite library with four different phosphobinding domains (14-3-3β, 14-3-3σ, the WW2 domain of NEDD4, and...68307 ) to generate the phosphosites with serine instead of phosphoserine (i.e. a “phosphorylatable” phosphosite...

...Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For multicistronic...different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene...different fluorescent tags for you to create fusion proteins with your gene of interest. These are organized... at high concentration, so this is noted here. Proteins that are derived from GFP and contain the A206K...information on the properties of many of these proteins, see: Cranfill et al. , Nature Methods 2016 Jul...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...