We narrowed to 116 results for: ANT;

... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the...bimolecular fluorescence complementation experiments in plants. The Zinc Finger Consortium Modular Assembly Kits...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...leads to slow bacterial growth, or maybe you only want to study the effects of protein expression in the...the stationary phase. In these cases, you may want to control protein levels or to turn on expression ...presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems also use... or introducing specific mutations can reveal important insights into gene function. These genetic changes...

...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...turning on another. The FLEx switch system takes advantage of the orientation specificity of Cre and the ...different types of target sites available, both mutant and wild type. The ability to manipulate the number... to genomic instability and cell death. It is important to incorporate appropriate controls such as Cre-only...

... by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA variant inactivated... genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal... is then recruited to the ribosome, where its anticodon can bind with the complementary codon on the mRNA...aaRS M. jannaschii sulfotyrosine (sY) Bacterial, Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine...inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS/tRNA pair instead. For directed...knocked out, tyrTV and tyrU genes replaced by tRNA variant that suppress the TAG nonsense codon). Expresses...

...CRISPR systems, the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems...CRISPR Plasmids: Cascade-Cas3 Mammalian Bacteria Plant Resources Cas3 is a unique member of the CRISPR ...double-strand break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound...Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return...

...Edit CRISPR Plasmids: Base Edit Mammalian Bacteria Plant Yeast Zebrafish Resources Two classes of base editors... base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements...fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window... Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due...Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses ...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID-19 and...

... new window) More Resources at Addgene: Recombinant Antibodies for Epitope Tags and Other Markers AAV ... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...over 100,000 unique combinations detectable by antibodies. All of the PRISM protein tags have been validated...corresponding primary and dye-conjugated secondary antibody (as listed in Park & Sheridan et al., 2025). These...

...Yeast Expression mGreenLantern 503 514 73 5.6 14 min Monomer (A206K) pcDNA3.1-mGreenLantern - Mammalian Expression... Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern - Yeast Expression Clover... is the product of exctinction coefficient and quantum yield divided by 1,000. Note that the effective...10His-mStayGold (E138D) - Bacterial Expression pJRA110 - Plant Expression mStayGold 499 510 136 4.8 Monomer pRSETB... to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to...

... to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from patient-specific...mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Mouse Direct reprogramming of fibroblasts into antigen-presenting dendritic cells. Sci Immunol. 2018 Dec...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...

...hormones or metal ions, scientists have adapted an antibiotic resistance operon from E. coli : the tetracycline...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...mutating the amino acid residues of TetR that were important for tetracycline-dependent repression led to a...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...#85972 for hygromycin selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber...

...other mutants Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR...eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection Disease...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ...

...designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed... for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans...tool for genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR ...

...protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical...proteins with disulfide bridges such as antibodies. MCP-tag is a mutant of ACP-tag, and is labeled by SFP Synthase...

... genome into a host cell. Scientists can take advantage of this to stably integrate transgenes of interest...Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest?...pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells. See the Weinberg...Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy, as well as... as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal... mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93...

...though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small...serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV9 AAV11 AAVrg Want more information before choosing a serotype? Learn... on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer plasmid...you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected] ....

...during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell... loss and ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. ...for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids...that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted...

...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...properties of the final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured...within representative vector preparations was quantified with electron microscopy after negative staining...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

...available as cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...