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  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ... your system, you can search or sort this table based on: Your particular expression system , eg. Mammalian... and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector...System Mammalian MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses. Can...Genome Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression...available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly of single... cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC vector. Uses five distinct zebrafish...

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...off-target effect). The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of...step-wise instructions on designing and using Cas9-based genome engineering tools to perform different types...express without the G. Because the G is only one base, the Zhang lab usually adds it when they order the...oligo from IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA...

  4. COVID-19 Resources

    Type
    Collection
    ...FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity... A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material. Rauch ...

  5. TALEN Guide

    Type
    Collection
    ... Figure 2 ). The Bogdanove group also hosts web-based software to help scientists find potential TAL effector...assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL...

  6. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  7. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  16. Fluorescent Protein Guide

    Type
    Collection
    ...backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein Fusion Construct...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...Galleries from Michael Davidson and FSU Spectra Database hosted at the University of Arizona Zeiss Education...

  17. AAV Viral Preps

    Type
    Collection
    ...Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples ...

  18. Plasmid Collections

    Type
    Collection
    ...Resources Our curated resources group plasmids together based on type or application. Browse our guides to find...

  19. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...degradation. This degradation can continue for many kilobases in one or both directions from the target region...
Showing: 41 - 60 of 95 results