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We narrowed to 118 results for: CAN

Showing: 41 - 60 of 118 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ..., allowing for precise gene editing. These edits can include mutations seen in human patients, protein...scalable. Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism....amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important to a... Nickase mutant: cuts only one strand of DNA and can be used with paired gRNAs to lower off-target cleavage...fused to transcriptional activators or repressors can modulate transcription in a reversible manner. Epigenetics...Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic...labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image genomic loci in live cells. RNA ...

  2. Mammalian RNAi Tools

    Type
    Collection
    ...dsRNA can be delivered as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur...similar mechanism. Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding...therapeutic targets, and delivery strategies for cancer therapy . International Journal of Nanomedicine...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...sequences are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve... nature of the disease, a small portion of cases can be attributed to known genetic factors such as defects...decision-making and language abilities. The disease can be classified as early-onset, where symptoms appear... of genetic modification and target species. You can also find our guide to using plasmid pooled libraries...protein, and more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid...

  4. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...of synthetic biology is the ease with which parts can be combined to construct complex networks. This is... which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest after...specific prefix and suffix sequences for each (these can be built into PCR primers when creating a new part...

  5. Adenovirus Plasmids

    Type
    Collection
    ...response induced by the viral capsid. Adenoviruses can be produced by several methods, the most common of... sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell... human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...distance between the donor and acceptor molecules and can therefore be used to calculate the distances between...intramolecular or unimolecular FRET). Fluorescent proteins can be used as donor and/or acceptor molecules in either...specific genes are also available. Custom biosensors can also be constructed using the cpFRET kit from the...Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein with...

  7. Rett Syndrome

    Type
    Collection
    ...spontaneously, although in rare cases Rett syndrome can be inherited. In females, random X inactivation results...mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2 protein is a global...mutation, the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly...Rett syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease...line , an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute:...backbones. A few examples of additional CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based...

  8. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce ...dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  9. Retrovirus Plasmids

    Type
    Collection
    ...retroviruses called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 ...Some disadvantages of γ-retroviruses are that they can only transduce dividing cells (this is because they...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis...

  10. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing. RGNs ...More information about RGNs and CRISPR-Cas systems can also be found at http://www.crispr-cas.org . Users...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...protein purification mentioned above, empty backbones can also be used for for viral vector delivery, genome...incorporated into the host DNA. For mammalian cells, you can do this through viral vector delivery. Visit...-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...Luciferase collection of plasmid backbones into which you can clone your regulatory element or gene of interest...regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly luciferase reporter...many plasmids have markers on them so that you can find or select for only the cells that received ...

  12. CRISPR References and Information

    Type
    Collection
    ...window) Identifies candidate gRNA target sequences in an input sequence, which can be an accession number... by amplicon sequencing. The updated CRISPresso2 can analyze outcomes from cleaving nucleases (e.g. Cas9...Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively selected sgRNAs...CRISPR MultiTargeter (Link opens in a new window) Can be used to identify novel gRNA target sites in a ...input sequence, checks for off-target binding and can work for S. pyogenes , S. thermophilus , or N. meningitidis...the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window...predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems...

  14. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...HDR). HDR is much less error-prone than NHEJ and can be used to faithfully introduce specific genomic ...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  15. Cre-lox system

    Type
    Collection
    ... Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre can excise the...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...expressing Cre at specific times or locations, you can precisely control expression of your gene of interest...recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Read...Cre expression construct for your experiment. You can search the table for the promoter, fusion, or expression...For more information on FLEx switch and what you can do with it read Addgene's Blog on FLEx Vectors . ...activates gene of interest by removing Stop sequences. Can be used for injection into fertilized zygotes Mammalian...

  16. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not... (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs can result in bidirectional insertions — meaning they...

  17. Genetic Code Expansion

    Type
    Collection
    ... or function in the protein can be observed. The introduced amino acid can also be used to intentionally... been introduced in the cells, the non-canonical amino acid can be incorporated using the existing protein...into the ribosome and if the anticodon on the tRNA can bind to the mRNA (hence, the anticodon is complementary...amino acid. Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the...thus this codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon...from M. jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution...light. On a broader scale, the expanded genetic code can help us understand and evolve proteins for various...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...regulatory elements before or after the luciferase ORF can be found in a table below, as well as plasmids that...are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection...collection of empty backbone plasmids into which you can clone your regulatory element or gene of interest... promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning...Luciferase driven by a strong constitutive promoter and can be used to monitor transfection or viral infection...firefly luciferase and dTomato. A gene of interest can also be inserted into the plasmid. Malcolm Moore ...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...

  19. Chemogenetics Plasmids

    Type
    Collection
    ...receptors are popular in neuroscience, where they can be used to interrogate brain function and examine...where the receptor is expressed, these receptors can be used to artificially activate or inhibit specific...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... dCas9-VPR for activation. Usage Guide sequences can be designed using Benchling or using similar design...containing the guide target sequence: Up to two guides can be expressed from the same plasmid by utilizing one...
Showing: 41 - 60 of 118 results