We narrowed to 101 results for: CAN

...This serine phosphosite library can then be used to identify candidate human substrates of kinases of ...labs at Yale University changed the way researchers can explore important questions surrounding serine phosphorylation...quantitative assessments. The Mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ...overexpression in E. coli . The phosphosite DNA library can also be introduced into a second expression vector...mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell sorting...Screening kinases The Mode #1 phosphosite library can also be synthesized using supD tRNA ( SupD (Plasmid...

...sequences are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve... nature of the disease, a small portion of cases can be attributed to known genetic factors such as defects...decision-making, and language abilities. The disease can be classified as early-onset, where symptoms appear... of genetic modification and target species. You can also find our guide to using plasmid pooled libraries...protein, and more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid...2006) Express human ATM with Flag and His tags. (Canman et al. Science. 1998) See More CRISPR Tools Find...

...of synthetic biology is the ease with which parts can be combined to construct complex networks. This is... which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest after...specific prefix and suffix sequences for each (these can be built into PCR primers when creating a new part...

...spontaneously, although in rare cases Rett syndrome can be inherited. In females, random X inactivation results...mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2 protein is a global...mutation, the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly...Rett syndrome-specific differentiated cells that can be used to understand the mechanisms of the disease...line , an immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute:...backbones. A few examples of additional CRISPR resources can be found below: Base Editing RNA Editing CRISPR-based...

...protein purification mentioned above, empty backbones can also be used for for viral vector delivery, genome...incorporated into the host DNA. For mammalian cells, you can do this through viral vector delivery. Visit...-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...Luciferase collection of plasmid backbones into which you can clone your regulatory element or gene of interest...regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly luciferase reporter...many plasmids have markers on them so that you can find or select for only the cells that received ...

...window) Identifies candidate gRNA target sequences in an input sequence, which can be an accession number... by amplicon sequencing. The updated CRISPresso2 can analyze outcomes from cleaving nucleases (e.g. Cas9...Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively selected sgRNAs...CRISPR MultiTargeter (Link opens in a new window) Can be used to identify novel gRNA target sites in a ...input sequence, checks for off-target binding and can work for S. pyogenes , S. thermophilus , or N. meningitidis...the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window...predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated...

...sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology...Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems...

...the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not... (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs can result in bidirectional insertions — meaning they...

...Photoactivatable iRFPs can be turned on by non-phototoxic far-red light and can be used for spatially ...

... dCas9-VPR for activation. Usage Guide sequences can be designed using Benchling or using similar design...containing the guide target sequence: Up to two guides can be expressed from the same plasmid by utilizing one...

...of transfer, packaging, and helper plasmids that can be used for AAV vector production suitable for a ...the transfer plasmids in Addgene's repository. You can search or sort this table based on plasmid name, ...separate helper plasmid during AAV production. You can search or sort this table based on plasmid name, ...

...few examples of useful zebrafish CRISPR resources can be found below: UFlip CRISPR targeted integration...studying cells that share clonal origin in zebrafish can be found below: Optimized QF-binary expression system...in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids for Zebrafish... translational research, including the study of cancer, inflammation and infection, regeneration, human... Zon Lab. A platform to rapidly model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...

...editors (CBEs) and adenine base editors (ABEs) — can be used to generate single base pair edits without...targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small editing...

...Characterization of some of the FPs in this collection can be found in Botman et al., 2019 . FP Tagging Yeast.... The FP together with the protothropic cassette can be amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA...

...serotype selection after you submit your request, so we can set expectations to the best of our knowledge from... three years of the order being shipped, Addgene can provide the QC validation data performed on the material...experiments carried out with our materials, Addgene cannot guarantee the function of any viral vector. Addgene...

...Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different serotypes...µL. The 20 µL option is an economical choice that can be used to validate the performance of various serotypes...

...provides a curated selection of popular plasmids that can be used for adenoviral vector production. Read our... human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction Bunz Return to...

...the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that express...Plasmids A few examples of reporter plasmids that can be used for measuring infectivity and neutralizing...

...fusion protein using fluorescence microscopy, you can assess whether your protein is targeted to the same...for chemical reactions. Labeling these structures can be quite useful for identifying the localization ...Neurons) Drd1 EGFP Kirk Mykytyn *Fusions to other FPs can be found by searching the complete Michael Davidson...domain mCherry Maria Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson...Occludens-1 EGFP Alan Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson...Rab11a DsRed Richard Pagano *Fusions to other FPs can be found by searching the complete Michael Davidson...

...described below. Information for a specific antibody can be found on the antibody's catalog page or by contacting...conjugation. For applications such as these, the buffer can be removed by dialysis or gel filtration. Titer and...