We narrowed to 103 results for: Nes
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TypeCollection...of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate...diverse set of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...
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Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs...):1471-1501. PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases... -
p53 Pathway
TypeCollection...in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid... phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper... -
TALEN Guide
TypeCollection... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...di-residue (RVD). Ultimately, it is this RVD that determines which single nucleotide the TAL effector will... Daniel Voytas’s group from the University of Minnesota, arrays can be assembled in a matter of days. ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove... -
Arf GTPase Family
TypeCollection...GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene...list of plasmids available. Please note that some genes may not yet have plasmids available for request.... GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each... -
Open Enzyme Collection
TypeCollection... Depositor Collections FreeGenes Project Open Enzyme Collection FreeGenes Project Open Enzyme Collection...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of ...Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...single nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets...The following plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate... -
KLF Research Plasmids
TypeCollection...Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...Addgene or email [email protected] to get started. Genes KLF gene pages with information on available plasmids... -
Michael Davidson Fluorescent Protein Collection
TypeCollection...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...tutorials, microscopy protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue... -
CRISPR Plasmids - Parasites
TypeCollection...(toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and ...10 guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ... -
Promega Plasmid Collection
TypeCollection... DNA size are processed and shipped within 6–8 business days. For more information about the ordering ... detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT... form an active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...plasmids (e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read...encoding AAV Rep and respective capsid protein which determines the AAV serotype) are used in conjunction with... -
Ras Pathway
TypeCollection...a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam K, Schreck... -
TALEN Plasmids and Kits
TypeCollection...PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD ...mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction ... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ... top vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging.... A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. Cell ... -
Zinc Finger Consortium Reagents
TypeCollection...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled... -
Fluorescent Protein Guide
TypeCollection...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Additional Resources at Addgene Addgene Blog:... -
Plasmid Collections
TypeCollection... Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi...