...Imaging More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background...variants have been shown to have high effective brightness and allow for single or multicolor imaging. Photo-activatable...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

...recombinase-expressing mouse lines 2-9 and combinations of transgenic recombinase-expressing animal lines and retro-grade...these ORF fragments (which have become exons) determines the logical expression requirements of Cre and...Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large...genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and...1351-1368. PMID:30755721 Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi Venkataraju...

... discriminate between positive and negative clones. Clones with insertion will show only linearized plasmid...FAQs Can you summarize HR template vector design guidelines? A few notes below are considerations for designing...plasmid of ~8.5 kb (only AgeI will be able to cut). Clones without insertion will show a ~1kb and ~7.5kb fragment...) on each colony in order to screen for positive ones. - Or - If your HR template has a selection marker... primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...

...binding platform, to repress expression of arbitrary genes in bacteria or human cells ( Qi et al. ). This CRISPR...juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared to several recently published...applying the wild-type CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , ... could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene... up to 2~3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR...

...Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants or browse...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 ...stem cells. Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral...from four separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming ...iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human ...

... induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use the ...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This...be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the following...

...pMJ825 : Streptococcus pyogenes Cas9 D10A mutation. 39316 pMJ826 : Streptococcus pyogenes Cas9 H840A. 39317...below: ID Plasmid 39312 pMJ806r : Streptococcus pyogenes Cas9. 39313 pMJ823 : Listeria innocua Cas9. 39314...meningitidis Cas9. 39318 pMJ841 : Streptococcus pyogenes Cas9 D10A, H840A. 41091 pHMGWA-Pa14Csy4 : Pseudomonas...

...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...lab fluorescent protein fusion constructs and backbones. Plasmid...Collection More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The...

...of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate...diverse set of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs...):1471-1501. PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases...

...GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene...list of plasmids available. Please note that some genes may not yet have plasmids available for request.... GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each...

...in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid... phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper...

... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...di-residue (RVD). Ultimately, it is this RVD that determines which single nucleotide the TAL effector will... Daniel Voytas’s group from the University of Minnesota, arrays can be assembled in a matter of days. ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove...

... Depositor Collections FreeGenes Project Open Enzyme Collection FreeGenes Project Open Enzyme Collection...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of ...Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification...

...Mammalian S. pyogenes Cas9 expression, human optimized 48645 DS-SPcas Bacterial S. pyogenes Cas9 (SP) +...bacteria. Cas9 proteins in human cells: Streptococcus pyogenes (SP): This is the classical Cas9 protein used ...performs poorly as a transcriptional repressor. All genes except the SP nuclease are human codon optimized...

...10.1186/s13104-018-3262-4. PubMed . Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis...Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573... Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520. doi: 10.1111/gtc.12492... ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. 2016 Apr;21(4):370-7. doi: 10.1111/gtc.12341...

...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...plasmids (e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read...encoding AAV Rep and respective capsid protein which determines the AAV serotype) are used in conjunction with...pRepCap6 AAV6 pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal...

...single nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets...The following plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate...

...Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...Addgene or email [email protected] to get started. Genes KLF gene pages with information on available plasmids...

... . These plasmids are described in: Targeted mutagenesis in the model plant Nicotiana benthamiana using...endonuclease. Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S. Nat Biotechnol . 2013 Aug 8;31(8):...23929340 . Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...