We narrowed to 76 results for: plasmid dna
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TypeCollection...Promega Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection are available...our help center . Plasmid Collection Browse and sort the table below to find plasmids in the collection...a new window) on Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...operational complexity. This unique collection contains plasmids developed by Promega scientists and designed to...HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega collection are available in two...
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CRISPR Pooled gRNA Libraries
TypeCollection...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system... -
CRISPR Plasmids - RNA Editing
TypeCollection...Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for RNA editing...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize...Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in... sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... -
TALEN Engineering
TypeCollection... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ...arrays on a single architectural framework and the DNAs produced by all three methods are identical in sequence... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287...S218TAG Plasmid 68300 MBP-MEK1 Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297...GFP Q157TAG Plasmid 68296 GFP S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ...68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid....ΔA.Δserb.Amp Plasmid 68305 Beta lactamase S68TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68301 MBP-MEK1...pSerOTS-C1* (V70) (Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important...-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1... -
Antibody Plasmid Collection
TypeCollection...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies are produced... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...expression vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through... Plasmid Collections Zinc Finger Consortium Zinc Finger ... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you... -
Luciferase Plasmid Collection
TypeCollection...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... Plasmid Collections Luciferase...Luminescent Imaging with Nano-lanterns Promega Plasmid Collection Luciferase is the enzyme responsible... -
Microbiology Resources
TypeCollection... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits...P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration of DNA into prokaryotic chromosomes - Endy... -
Plant Plasmids and Resources
TypeCollection... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...resources: Find plasmids for use in plants. Plasmid... Background Addgene’s repository contains many plasmid tools that were specifically designed for use in...use in plants. The tables below highlight plasmids spanning a wide variety of molecular biology technologies...fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell... -
Bacterial Expression Systems
TypeCollection...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression ...CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR Plasmids page ... -
Neurodegeneration Research Collection
TypeCollection... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi... -
CRISPR References and Information
TypeCollection...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:... -
Genetic Code Expansion
TypeCollection...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate the ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin... Farren Isaacs Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All... -
Zhang Lab CRISPR Page
TypeCollection...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome... -
Tetracycline Inducible Expression
TypeCollection...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard...expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...