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Showing: 31 - 40 of 123 results

  1. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

  2. Institute for Protein Innovation

    Type
    Collection
    ... Glypican Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Integrin... Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Browse...express plasmid-based antibodies. ID Plasmid Description...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

  5. Qi Lab CRISPR Page

    Type
    Collection
    ...3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR as an...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically inactive...

  6. Control AAV Preps

    Type
    Collection
    ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more....Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... and Eric Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast... M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel B. Physiol Genomics . 2002 Dec 3;11(3):...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...

  10. Chemogenetics Plasmids

    Type
    Collection
    ...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...inhibit the target neuron. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you...
Showing: 31 - 40 of 123 results