We narrowed to 74 results for: RAN-1

... your account. We will notify you by email within 1 business day regarding your account verification. ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

... find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...

...available as a substrate for another round. Figure 1: Overview of prime editing. Created with BioRender.com...incorporated into the DNA by reverse transcription. The edited DNA strand displaces the unedited 5’ flap and...replace” gene editing method in which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A...the target sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the...Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components...including exploring new sources for the reverse transcriptase. Many PE enzymes and related tools are available...

...serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing Rep2 and Cap1...use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene or genetic elements of...service gives you even more options. AAV Transfer Plasmids Transfer plasmids encode the genetic information...Browse AAV transfer, packaging, and helper plasmids in Addgene's repository. Viral...Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids...precise delivery of genetic material across a wide range of tissue types with minimal immune response in ...This collection provides a curated selection of transfer, packaging, and helper plasmids that can be used...

...component involved in GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid...2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular ...., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi,...

...coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease..., that will probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):149-53. PMID...

...to determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...cell cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and ...section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...opens in a new window) ). Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector,... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...

...one of three different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases...deposition with APOE2 constructs . (Hudry et al. Sci Transl Med. 2013) Express human DNMT1 with a Myc tag. ...vesicular brain cells and more. Measure synaptic transmission with improved glutamate indicators . (Aggarwal...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors for rapid and efficient generation of...astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...

...infection to host cells . Signal transduction and targeted therapy, 5 (1), 283. https://doi.org/10.1038/...envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for protein production...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function (Bosch et al., 2008). TACE plasmids... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled...

..., 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9...sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed...gene inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants,...and cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants,...Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate CRISPRko/a/i sgRNA sequences...for off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...

...and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made... together to form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for...proteins by size. Transfer proteins from the gel to a membrane. Incubate the membrane with an unconjugated...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies....variable regions, shown in light blue and light orange, that bind to a specific antigen, triggering an...

...Control Viviana Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

... Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...can be used to study processes beyond transcription and translation. Splicing reporters from Younis et al...bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have made luciferase...Regulatory elements such as promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can... protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal protein ...

...Tools Atlas (Link opens in a new window) . Figure 1: Selection, prep, and testing of AAV enhancers in ...Tasic, B. (2025). A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell...