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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 138 results for: form

Showing: 81 - 100 of 138 results

  2. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the...Please click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970...(CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When using the AAV2(Y444F) serotype...

  4. Control AAV Preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...

  5. Lentivirus Plasmids

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer...

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...described below. Read our CRISPR 101 post for more information about HDR/repair template design. How to use ...

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Protein phosphorylation is one of the most abundant forms of posttranslational modifications in cells and ... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...Plasmids and Optogenetics Guide page for more information about this field. Use the table and filters below...

  10. Retrograde AAV viral preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...window) ) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your...pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in future publications...

  12. CRISPR Guide

    Type
    Collection
    ...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3 for more details...with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease domains...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an active,...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA. (4...multiple plasmids used for various platform applications. For more information on CRISPR, check out Addgene’...work to develop a highly specific, programmable platform well-suited for various biological and translational...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...function. This is due to the high frequency of indels formed on non-deleted alleles 2 , which are likely to ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR...ligation to occur in one reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting... cell type used at limiting dilution prior to performing this step to reliably obtain approximately 30...genomic DNA isolation may be utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ...activate all known coding isoforms form the RefSeq database (23,430 isoforms) for gain-of-function screening...mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 vector...et al ., Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol...the Addgene GeCKO Library CRISPR page for more information. Full references are below. Return to top Cas9...screening. For more information, please visit the Addgene SAM library page . Targeted gene activation using...recombinase-2A-EGFP-KASH Detailed backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB ...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Plasmids . For more information on stem cells, visit the NIH Stem Cell information page. Reprogramming...and then select the article link to find more information. Delivery Method Species Description Article ...and then select the article link to find more information. Convert From Convert To Delivery Method Species...

  19. Biosensor AAV Preps

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...
Showing: 81 - 100 of 138 results