...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...myristoylation signal For more information about tags, including amino acid sequences, see...delivery. Visit our viral vector page for more information. Below are some common delivery methods: Delivery... host genome. View our AAV page for more information. pAdTrack-CMV - Shuttle vector...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... AAV production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral...plasmids encode the AAV genome (i.e., the genetic information that will be contained in the virion) and/or ...

...hort P alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been... . Once expressed, the Cas9 protein and the gRNA form a ribonucleoprotein complex through interactions...protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex, which is delivered to cells...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...inactive, non-DNA binding conformation into an active DNA-binding conformation. Importantly, the spacer...positively-charged grooves on Cas9. Cas9 undergoes a conformational change upon gRNA binding that shifts the molecule...permit target cleavage. Cas9 undergoes a second conformational change upon target binding that positions the...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...enterobacterial genomes. The bacteria of interest are transformed with the appropriate construct, grown at the ...inside cells just isn’t enough to get all the information you need. If you want to dissect an individual...to the periplasm which may aid disulfide bond formation or prevent toxicity. Compatible with Gateway cloning...for protein localization to aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids with...Reporter system for testing hetero- and homo-dimer formation. Proteins of interest are fused to AraC or a dominant... from the araBAD promoter whereas heterodimer formation with dominant negative AraC decreases GFP expression...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...maximum of NanoLuc to that of the fluorophore. Conformationally destabilized Firefly luciferase mutants : ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0...

...individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA. (4...multiple plasmids used for various platform applications. For more information on CRISPR, check out Addgene’...work to develop a highly specific, programmable platform well-suited for various biological and translational...

...function. This is due to the high frequency of indels formed on non-deleted alleles 2 , which are likely to ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR...ligation to occur in one reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting... cell type used at limiting dilution prior to performing this step to reliably obtain approximately 30...genomic DNA isolation may be utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the...

...activate all known coding isoforms form the RefSeq database (23,430 isoforms) for gain-of-function screening...mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 vector...et al ., Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol...the Addgene GeCKO Library CRISPR page for more information. Full references are below. Return to top Cas9...screening. For more information, please visit the Addgene SAM library page . Targeted gene activation using...recombinase-2A-EGFP-KASH Detailed backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Plasmids . For more information on stem cells, visit the NIH Stem Cell information page. Reprogramming...and then select the article link to find more information. Delivery Method Species Description Article ...and then select the article link to find more information. Convert From Convert To Delivery Method Species...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... is mutation is noted when present. For more information on the properties of many of these proteins, ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...also listed here and denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...switch a powerful experimental tool. For more information on FLEx switch and what you can do with it read...technology. Read the genOway press release for more information. Empty backbones to regulate your gene of interest...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...specific molecules or other factors. Most often, a conformational change in the sensing region of the construct...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...

...subunit B, beta isoform PPP3RL PRF1 perforin 1 (pore forming protein) FLH2, HPLH2, MGC65093, P1, PFN1,...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B),...PPP3CA protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, ...PPP3CB protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein...

...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...Link opens in a new window) . Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...

... to resources and information. Researchers can obtain the desired modules to perform their functional ...available modules, as well as find information on the Casilio platform. Protocols & References Casilio: ... PubMed PMID: 26768771 . Casilio platform website : Find information and resources on using Casilio, as...Jackson Laboratories. Next generation CRISPR-Cas platform for multiple applications. Genome...Jackson Laboratory (JAX) have developed a CRISPR/Cas platform called Casilio , which is a hybrid system combining...sequences and modules JAX press release on the Casilio platform Casilio Plasmids Cas9 plasmids For Cas9, use a...

...methods, and quality control are described below. Information for a specific antibody can be found on the antibody's...reviewing the specific antibody webpage for further information. Production For recombinant antibodies (rAb) ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the.... To learn which specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry...

...bio 73098 CD44-isoform1-CD4d3+4-bio 73099 CR1-isoformB-CD4d3+4-bio 73100 Basigin-isoform1-CD4d3+4-bio 73101...Galectin3-CD4d3+4-bio 73129 Basigin-isoform2-CD4d3+4-bio 73130 CD44-isoform12-CD4d3+4-bio... Please see the following reference for more information: Basigin is a receptor essential for erythrocyte...

...literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information (Link...which they were created. Find their contact information by following the link to their laboratory website...Link opens in a new window) Wendy Gold For more information on generating iPSCs using plasmids, please see...MECP2 - MECP2 curated protein and functional information Mouse and Cell Line Repositories (Link opens ...Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH image data...

...economical choice that can be used to validate the performance of various serotypes before conducting larger...larger scale experiments. For more information, see our full AAV inventory or our AAV production page . Note...direct GFP expression from the CAG promoter. For information about each catalog item, including the titer ...expression from the human synpasin promoter. For information about each catalog item, including the titer ...know what you would like to see available in viral format. Please note this does not guarantee viral service...