...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish...Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous human...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian cells...human genes Guide RNA expression plasmids for endogenous zebrafish genes...

...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express... central phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein...

...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid...MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett...mammalian CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few...gene. Use the search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial...

...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...electrical potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information... ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Dimidschstein 137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2...eYFP E2 C1V1 EYFP Constitutive 1, 5, 9, PHPeB Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs...Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick ...CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for each ...

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine...GCaMP6f E2 GCaMP6f none Constitutive 1, 9, PHPeB Dimidschstein 137122 pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11...ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG...group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone receptor PPARA Plasmids PPARA, SC22CB-5E3.5,...receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1, ROR2...orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid...collection for the community. If you have created plasmids that you would like to add to this collection,...NR3C4, SBMA, SMAX1, TFM androgen receptor ESR2 Plasmids ESR2, ER-BETA, ESR-BETA,ESRB, ESTRB, Erb, NR3A2...

...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...are usually divided among many plasmids, such that all of these plasmids must be present for a viral particle...vector plasmids available at Addgene and other related resources. Special...

...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab... Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR ...Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse...involved in defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene’s repository ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or check ...out our additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute...

... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...Your Perfect Plasmid Backbone Empty backbones are plasmids containing only the elements needed for replication...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent...marker Also see more empty backbones on our Plant Plasmids and Resources collection page Yeast GAL4, PGK,...

...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids - Enhanced...C-terminal GFP for bacterial expression Davidson Lab Plasmids - Includes many fluorescent proteins in ...FRET Biosensors Subcellular localization Michael Davidson Collection Blog: Which FP Should I Use? Blog: ...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...

...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ...Collections Addgene's repository contains 158,781 plasmids . We've gathered our curated plasmid resources...Expression Worm Expression Kits Kits are groups of plasmids that have been packaged together because they ...Pooled Libraries Pooled libraries are a set of plasmids all built with the same backbone and only differing...

...mTORC1 Activity in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids; these are noted with the viral service icon . Metal Ions...Neurosci. 2020 Dec;23(12):1629-1636. Jordane Dimidschstein Calcium Calcium sensor in astrocytes (GCaMP6f...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin ...

...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...archaea use to protect against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety ...CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can ... (see: Validate genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome...

...Fluorescent Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP...like transcription. Michael Davidson FP Collection Browse the Michael Davidson comprehensive collection ...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists...repository contains a variety of fluorescent protein plasmids. Use this guide to learn more about the many applications... of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing scientists... scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization...genetically encoded fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control...

...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...components. Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic...level gene networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental...including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian...

... empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600...Plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins....imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/...Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation) 3.2 pPAiRFP1... pPAiRFP2-N1 Do you have suggestions for other plasmids that should be added to this list? Fill out our...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...Synthetic Biology plasmids for cloning and genomic tools. Plasmid...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table... Back to Top Do you have suggestions for other plasmids that should be added to this list? Fill out our...