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Showing: 101 - 120 of 154 results

  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together .... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are ...more plasmids containing the necessary components of the viral genome to assemble the viral capsids (packaging...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...information to cells. These vectors rely on production plasmids that have been carefully optimized for both safety...vectors and find viral production and transfer plasmids for a wide array of research purposes. Interested...

  3. Plasmid Collections

    Type
    Collection
    ...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ...Collections Addgene's repository contains 165,187 plasmids . We've gathered our curated plasmid resources...Expression Worm Expression Kits Kits are groups of plasmids that have been packaged together because they ...Pooled Libraries Pooled libraries are a set of plasmids all built with the same backbone and only differing...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Addgene Blog: Biosensors Luciferase Plasmids Optogenetics Plasmids Viral Service: AAV Biosensors Fluorescent...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more. Metal Ions ...Neurosci. 2020 Dec;23(12):1629-1636. Jordane Dimidschstein Calcium Calcium sensor in astrocytes (GCaMP6f...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin ...

  5. CRISPR Guide

    Type
    Collection
    ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...archaea use to protect against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety ...CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can ... (see: Validate genetic modification ). Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1...Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3...Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 h Monomer...Emission (nm) Brightness pKa Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1...Emission (nm) Brightness pKa Maturation Structure Plasmids mCrimson3 588 615 Monomer mCrimson3-N1 - Mammalian...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids that provide Cas9...gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas protein act...of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a...single CRISPR function. Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9...

  8. Synthetic Biology - Overview

    Type
    Collection
    ...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...components. Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic...level gene networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental...including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian...

  9. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...Collections Synthetic Biology SynBio Plasmids Synthetic Biology: SynBio Plasmids SynBio Resources SynBio Home ...list of available plasmids related to synthetic biology below. If there are plasmids you would like to...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....please email [email protected] . Synthetic Biology Plasmids Search the table by keyword or sort by the table...

  10. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...Synthetic Biology plasmids for cloning and genomic tools. Plasmid...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table... Back to Top Do you have suggestions for other plasmids that should be added to this list? Fill out our...

  11. Fluorescent Protein Guide

    Type
    Collection
    ...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty.... Michael Davidson Fluorescent Protein Collection Browse the comprehensive Michael Davidson collection...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists...Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains... biosensors. Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and control molecular... of cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate or inhibit genetically...

  12. Neurodegeneration Research Collection

    Type
    Collection
    ...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... 4. See More iPSC Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons.... Lam et al. bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription...APP processing and dynamics using APP and Bace1 plasmids . Capitini et al. iScience. 2023 Apr 11. See More...research community. This collection highlights plasmids created with support from the BRAIN Initiative...

  13. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...from the Michael Davidson Lab.... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein...Protein Collection Michael Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald...Orange Red Photoactivatable The lab of Michael Davidson from Florida State University has contributed ...localization data found below. In addition, the Davidson lab has contributed the corresponding empty backbone...proteins with your gene of interest. Most of these plasmids are available to industry scientists as well as... more about this collection, or visit Michael Davidson's Molecular Expressions website (Link opens in ...

  14. Depositor Collections

    Type
    Collection
    ...Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors ...expression, synthetic biology, and viral vector plasmids. Plasmid...Collections Addgene's repository contains 165,187 plasmids, which includes plasmid collections from depositors...TREAT-AD Plasmid Collection Visualization Michael Davidson Fluorescent Protein Collection Allen Institute...

  15. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Excitation/Emission Brightness Plasmids mCardinal 604/659 18 mCardinal plasmids from Chu et al. (2014) mNeptune...Find plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins...imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Near-Infrared Protein Excitation/Emission Brightness Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/...Photoactivatable Protein Excitation/Emission Brightness Plasmids PAiRFP1 690/717 (after photoactivation) 3.2 pPAiRFP1... pPAiRFP2-N1 Do you have suggestions for other plasmids that should be added to this list? Fill out our...

  16. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21...KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF... lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled...

  17. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...fluorophore) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR...mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you ...options. Browse Addgene's collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are...You then delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar...

  18. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m...mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth factors... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...: 25743081 . Do you have suggestions for other plasmids that should be added to this list? Fill out our...

  19. TALEN Guide

    Type
    Collection
    ...Guide to TALEN technology and using TALEN plasmids...Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in...repeats only differ from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately... in a matter of days. In an open access Nucleic Acids Research article that came out in the spring of ... 2011, the groups describe a set of customized plasmids that can be used with the recently developed Golden...ordered fashion in a single reaction.” Using these plasmids, which are available as a kit from Addgene , custom... JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(12):e82. Epub 2011 Apr 14. Pubmed...

  20. Synthetic Biology - Algal

    Type
    Collection
    ...collection of synthetic biology plasmids for use in algae. Algal Plasmids Search the table by keyword or...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...Browse Addgene's collection of synthetic biology plasmids for use in algae. Plasmid... Publication Do you have suggestions for other plasmids that should be added to this list? Fill out our...
Showing: 101 - 120 of 154 results