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  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...for gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral...for yeast expression pET GFP - C-terminal GFP for bacterial expression pPD95_75 - C-terminal... shRNA expression MSCV-IRES-GFP - Mammalian retroviral gene expression with GFP selectable...Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pcDNA3 GFP LIC cloning ...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with... expression - Gradia Lab plasmids for mammalian expression, Ligation Independent... plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids for drosophila...

  2. Brain Initiative Collection

    Type
    Collection
    ...IRES EGFP Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the...83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic...83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic...83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic...83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic...83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic... Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  3. Cre-lox system

    Type
    Collection
    ...48201 CAG-GFP-IRES-CRE Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG...116879 CAG-Cremyc-2A-GFP GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral...dependent DsRed expression Mammalian Cepko 13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko...pAAV-FLEX-GFP Cre dependent GFP expression AAV Boyden 37402 Cre Stoplight 2.4 Zsgreen is expressed in cells... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System...iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler...

  4. Retrovirus Plasmids

    Type
    Collection
    ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro...also expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also ...MSCV-IRES-GFP MSCV For transgene expression and GFP marker Reya 21654 pMSCV PIG (Puro IRES GFP empty plasmid...fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also ... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...

  5. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block for ...viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...

  6. Brain Armamentarium

    Type
    Collection
    ...Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... BRAIN Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein ...Boyden 37825 pAAV-CAG-GFP CAG GFP Control Boyden 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 ...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2....hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...

  8. Retrograde AAV viral preps

    Type
    Collection
    ... pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell... PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control...mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP...EGFP-tagged Cre expression Recombinases Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre...Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson...CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous...simultaneous dTomato expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral...Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - Bacterial Expression mAzamiGreen 492 505...Mammalian Expression pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6...pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ...Mammalian Expression mEmerald-C1 - Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...of interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...gene expression at a single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under...option rtTA On Kowarz 16623 pBI-GFP Expression of your gene of interest & GFP from a bidirectional tet-responsive...pTet-IRES-EGFP Lentiviral plasmid for inducible expression of transgene of interest and EGFP None Either... and additional plasmids tetR-KRAB On Aebischer & Trono 16542 pBI-MCS-EGFP Expression of your gene of ...

  11. Bacterial Expression Systems

    Type
    Collection
    ...Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification Controlled Expression...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. ... on expression only at a specific time. This collection has a variety of plasmids whose expression levels...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...drive gene expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used...studying gene expression in Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (...Overview of plasmids used for expression in bacterial systems Plasmid...

  12. AAV Molecular Tools

    Type
    Collection
    ...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118... ablation. ID Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent...Cre-dependent Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation...viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent... ID Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...Cre-dependent Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals...pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound...

  13. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ... Investigator Plasmids GFP GFP-dependent transcription factors Connie Cepko See Plasmids Dronpa Optical...take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein Purpose...activity Michael Lin See Plasmids Do you have suggestions for other plasmids that should be added to this...Plasmids using a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression... expression. Plasmid... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity...Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, but new methods...

  14. Mammalian RNAi Tools

    Type
    Collection
    ... shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended...shRNA expression ID Plasmid Description PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob ...Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes EGFP to be recombined...shRNA libraries Lentiviral packaging and expression plasmids Background RNA interference (RNAi) is an ...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...Lentiviral vectors for Tet-regulated shRNA expression ID Plasmid Description Tet on or off PI 11643 pLVCT-tTR-KRAB...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP S2TAG...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ...S2TAG/E17TAG Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO...

  16. Lentiviral Prep Service

    Type
    Collection
    ...17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter... 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 ...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ...to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and ...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...

  18. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes reported...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ...the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from ...

  19. Control AAV Preps

    Type
    Collection
    ...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10...*, PHP.eB, PHP.S Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB ...dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Fishell 83894...PHP.eB Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP Constitutive 1, 9, PHP.eB Dimidschstein... 9 Wilson 105598 pAAV.GfaABC1D.PI.Lck-GFP.SV40 GfaABC1D Lck-GFP Constitutive 5 Khakh 105622 pAAV.CamKII...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...

  20. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear...
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