Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 16 results for: cre dependent fluorescent reporter

Showing: 1 - 16 of 16 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...Edward Boyden Voltage Fluorescent voltage reporter Voltron (Constitutive or Cre-dependent) Bright and photostable... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal ...photoconvertible fluorescent protein-based calcium integrator (CaMPARI) (Constitutive or Cre-dependent) Neural ... AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...

  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...find other Cre-, Flp-, or Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids ...luciferase and Cre to interrogate tumorigenesis in vivo using a mouse line expressing Cre-dependent Cas9. Kits...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre ... Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific...expressing Cre , Flp , Dre , other recombinases , as well as useful empty backbones or reporters . Experimental... interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent gene expression ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...

  3. Brain Initiative Collection

    Type
    Collection
    ...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter...AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP Viviana Gradinaru...AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...

  5. Biosensor AAV Preps

    Type
    Collection
    ...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1 Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent 1 Podgorski...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent...none Cre dependent 8 Deisseroth 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth...

  6. Bacterial Expression Systems

    Type
    Collection
    ... Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2...ratio Fluorescence (mCherry) Mycobacterium sp. Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Expression Constructs Reporter Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc... element or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids...pC0037 Gaussia, Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina...imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins ...Region) : Genome-wide screening of enhancers using a firefly luciferase reporter. These plasmids improve...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...

  9. Viral Production

    Type
    Collection
    .... Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  10. COVID-19 Resources

    Type
    Collection
    ...virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice, Ts1Rhr . International...host cell, like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular...acids SARS-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase..., & Pöhlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically...Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics Institute...), including plasmids for protein production, secretion, and codon-optimized versions designed...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions Figure...proteome-wide identification of phosphorylation-dependent interactions . Nature Biotechnology , 36 (7), ...domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also...

  12. Plasmids for Stem Cell Research

    Type
    Collection
    ... adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8)...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion of Fibroblasts. Neuron. 2014...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible... evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet...85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji...63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris...Repressible Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids...a minimal CMV promoter, and other tet- or dox-dependent promoters (sometimes generally called Ptet) have...expression. Tetracycline Off is also known as the tTA-dependent or tet-repressible system. Tetracycline On (Tet-On...

  14. CRISPR Guide

    Type
    Collection
    ...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA...have also led to reduced dependency on specific PAM sequences and the creation of new tools. Figure 3: ...binding signal for Cas9, but the exact sequence depends on which Cas protein you use, and there are several...homology arms). The length of each homology arm depends on the size of the change being introduced; larger...larger insertions require longer homology arms. Depending on the application, the donor DNA may be a single-stranded...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated end-joining), an...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell..._CEBPA_1 PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...genome modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...
Showing: 1 - 16 of 16 results