...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...Edward Boyden Voltage Fluorescent voltage reporter Voltron (Constitutive or Cre-dependent) Bright and photostable... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal ...photoconvertible fluorescent protein-based calcium integrator (CaMPARI) (Constitutive or Cre-dependent) Neural ...(Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP A fluorescent, reagentless biosensor... AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr...

...Rho-Cre is expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables...-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (...recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion...pOG231 NLS-Cre CMV Mammalian Wahl 19131 pIC-Cre Cre Bacterial Rajewsky 22776 MSCV CreERT2 puro Cre-ERT2 - ...mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft...comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool, Cre expression can...pPGK-Cre-bpA Cre PGK Mammalian Rajewsky 11916 pBS185 CMV-Cre Cre hCMV Mammalian Sauer 11918 pBS513 EF1alpha-cre Cre...

...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter...AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP Viviana Gradinaru...AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1 Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent 1 Podgorski...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent...none Cre dependent 8 Deisseroth 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth...

...virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV...Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca 2+] i Increase in Down Syndrome Mice, Ts1Rhr . Do you...SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...

...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...

.... Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

...Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, ...them useful as transcriptional reporters. However, they are not secreted, so cells must be lysed or the...decay reporter and control plasmid utilizing Firefly luciferase. Transcriptional Element Screening Optimized...plasmids : Genome-wide screening of enhancer activity using a Firefly luciferase reporter. These plasmids improve... element or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids...transcriptional regulation and bioluminescent reporters. Plasmid...Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...

...split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent protein-peptide...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions. The...proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV...NEDD4-2; 111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also...

...evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495...the TRE, permitting transcription. Tetracycline-dependent promoters are developed by placing a TRE upstream...system. Tetracycline off is also known as the tTA-dependent system. Tetracycline On System In 1995, Gossen...residues of tetR were important for tetracycline-dependent repression. Mutating these residues led to the...on system is also known as the rtTA-dependent system. Experimental Tips Choosing a tet system If your gene...system are: Plasmid containing a tetracycline-dependent promoter upstream of your gene of interest tTA...system are: Plasmid containing a tetracycline-dependent promoter upstream of your gene of interest rtTA...

... adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8)...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion of Fibroblasts. Neuron. 2014...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...

...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA...have also led to reduced dependency on specific PAM sequences and the creation of new tools. Figure 3: ...binding signal for Cas9, but the exact sequence depends on which Cas protein you use, and there are several...homology arms). The length of each homology arm depends on the size of the change being introduced; larger...larger insertions require longer homology arms. Depending on the application, the donor DNA may be a single-stranded...

...elegans expression Other Fluorescent Proteins Localization See full Fluorescent Protein Guide...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...promoter strength pBV-Luc - Luciferase reporter plasmid with very low basal activity shRNA/RNAi...Hynes Lab Plasmids - A system for Cre-regulated RNAi in vivo Dupuy Lab and...mammalian expression, Ligation Independent Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-... from the SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription...

...C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated end-joining), an...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell..._CEBPA_1 PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...genome modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in...

... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning...TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...

...transfection conditions for each cell line with a reporter construct to ensure robust plasmid delivery before...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...incubate at 37 °C for 7 - 14 days. Vary these times depending on the doubling time of the cell line used. NOTE... Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines. Along... pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a locus in order...strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located within exons...strand ( Figure 2A ). However, DSB will occur independent of orientation of the protospacer sequence/PAM...