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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 5 results for: ena/1000

Showing: 1 - 5 of 5 results

  1. Antibody Guide

    Type
    Collection
    ...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

  2. Bacterial Expression Systems

    Type
    Collection
    ...Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered strain...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous cytoplasmic...pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification... cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors... repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that vary... . The Marionette Sensor Collection (Addgene #1000000137) allows you to independently control the expression...

  3. CRISPR Guide

    Type
    Collection
    ...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S.... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., ...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation... used for single-strand annealing assay, which enables the evaluation of TALEN activities in cultured ...-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed for ...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Alzheimer's Zena Werb 19138 mADAM10 deltaMP in pQCXIP cFLAG ADAM10 Flag Alzheimer's Zena Werb 19228 pBS...ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV ALS Yardena Samuels 29529 pcDNA3.1...E452K) ERBB4 CMV ALS Yardena Samuels 29530 pcDNA3.1-ERBB4 (E542K) ERBB4 CMV ALS Yardena Samuels 29531 pcDNA3.1...R544W) ERBB4 CMV ALS Yardena Samuels 29532 pcDNA3.1-ERBB4 (E563K) ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1...dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1-ERBB4 (E836K) ERBB4 CMV ALS Yardena Samuels 29535 pcDNA3.1...(E872K) ERBB4 CMV ALS Yardena Samuels 29536 pCDF1-ERBB4 WT ERBB4 CMV ALS Yardena Samuels 29537 pCDF1-ERBB4...E317K) ERBB4 CMV ALS Yardena Samuels 29538 pCDF1-ERBB4 (E452K) ERBB4 CMV ALS Yardena Samuels 29539 pCDF1...
Showing: 1 - 5 of 5 results