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We narrowed to 14 results for: lentiviral construct

Showing: 1 - 14 of 14 results
  1. Luciferase Plasmid Collection

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    ...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla...introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase Eric Campeau...
  2. Lentivirus Plasmids

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    ... safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids ...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...Addgene lentiviral packaging, envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...for safe use in the lab. One safety feature of lentiviral vectors is that the components necessary to produce...highlights popular second- and third-generation lentiviral packaging, envelope, and transfer plasmids available...
  3. Tetracycline Inducible Expression

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    ...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA...rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article...rtTA-Advanced H1-2O2 Stephen Elledge 111177 LT3GEPIR Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant...TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP...
  4. Viral Production

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    ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...and probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular...Identity Confirmation and Sterility All of our lentiviral vectors are confirmed to have the correct integrated...
  5. Lentiviral Prep Service

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    ...-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...available in lentiviral format....and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding... CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau...
  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression...
  7. Neurodegeneration Research Collection

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    ...Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer...structure and function with siRNA-resistance SigmaR1 constructs . (Sawyer et al. Dev Cell. 2024) Image exocytosis...Initiative Collection . Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP,...Cell. 2017) Study amyloid deposition with APOE2 constructs . (Hudry et al. Sci Transl Med. 2013) Express...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More...
  8. COVID-19 Resources

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    ...expression, and spike protein constructs for making VSV pseudo virus. Addgene blog Scientists...Reverse transcriptase, protease, and polymerase constructs for SARS-CoV-2 detection assays Neutralizing ... lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee...
  9. CRISPR References and Information

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    ...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new... Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection and ...triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal...
  10. Fluorescent Protein Guide: Empty Backbones

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    ...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression...Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct Kit Neveu Lab MXS Chaining Kit Lindquist Lab Yeast...
  11. CRISPR Guide

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    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...
  12. Fluorescent Protein Guide: Biosensors

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    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...
  13. Immunology Research Plasmids and Resources

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    ...flexible system that can be used to construct complex lentiviruses for modification of mammalian cells...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...-chaining system allows efficient assembly of constructs that are optimized for synthetic biology applications...
  14. Neurodegeneration Plasmid Collection

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    ...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...
Showing: 1 - 14 of 14 results