...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-...-iCre copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...switch CMV Mammalian Livet loxP (floxed) Constructs These constructs allow for Cre-regulated gene expression...Depending on the construct, Cre may activate or repress gene expression. Common construct types include:...

...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible...addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive...promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly luciferase fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly...Firefly luciferase fusions using Gateway cloning. Lentiviral. Mikko Taipale 87789 pPTluc Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 106292 pLS-SV40-mP-Rluc Renilla ...

...for additional constructs TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses ...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert and induce your ...On or Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning...insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene ...tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA...

...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...table lists lentiviral transfer plasmids that can be used with 2nd and 3rd generation lentivirus technologies...

...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...and probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular...Identity Confirmation and Sterility All of our lentiviral vectors are confirmed to have the correct integrated...

...-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...available in lentiviral format....and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding... CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau...

...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI ...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI...62348 Mammalian/Lentiviral BbsI none S. pyogenes Puro Ochiai pSB700 64046 Mammalian/Lentiviral none S. pyogenes...57822 Mammalian/Lentiviral BsmBI none S. pyogenes EGFP Ebert pSECC 60820 Mammalian/Lentiviral BsmBI yes, cut...

... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression...

...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus ...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...

...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new... Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection and ...triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal...

...Synuclein, and more. New and Noteworthy: Use tau constructs to study aggregation. Saha et al. Nat Commun.... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...

...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...

...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...

...flexible system that can be used to construct complex lentiviruses for modification of mammalian cells...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...-chaining system allows efficient assembly of constructs that are optimized for synthetic biology applications...

...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...28203 tdp43-EGFP construct10 TARDBP GFP CMV ALS Zuoshang Xu 28204 tdp43-EGFP construct11 TARDBP GFP CMV ...