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We narrowed to 14 results for: lentiviral production

Showing: 1 - 14 of 14 results

  1. Viral Production

    Type
    Collection
    ...Service Viral Production Viral Production Jump To: Viral Production Overview AAV Production Titer Quality...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is... to generate high quality preparations. After production, all virus preps are titered and subjected to...

  2. Lentivirus Plasmids

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    Collection
    ...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...encode a wide range of gene products. The main restrictions on a lentiviral transfer plasmid are size (...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...fusion protein. This means that the final product should be translated as a single string of amino...C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in E. coli...

  4. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...genetic information to cells. These vectors rely on production plasmids that have been carefully optimized for...different types of viral vectors and find viral production and transfer plasmids for a wide array of research...Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–130 nm Dividing/Non-dividing...vitro cell cultures, these plasmids direct the production of viral vectors that contain the desired genetic...

  5. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...modifications may enhance infectious particle production, including codon optimization, deletion of the...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase...luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly...

  6. Kazuhiro Oka Lentiviral Vectors

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    ...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid ...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  7. Viral Prep Service for Plasmids and Pooled Libraries

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    ... Vector Packaging Service Information Production Our production process and quality control, with examples... in our collection. We know that viral vector production is a common step after requesting plasmids from...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...Articles Additional details about viral vector production at Addgene, ordering information, and more. Additional... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

  8. Adenovirus Plasmids

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    Collection
    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...expression cassette. Bunz 16402 pShuttle Shuttle For production of viruses containing transgene under a chosen...promoter Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter...promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene...promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene... plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene...under CMV Bamburg 50958 ShuttleNSE Shuttle For production of viruses containing transgene under Neuron-...

  9. Luciferase Plasmid Collection

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    Collection
    ...interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla...21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase Eric Campeau... pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau... 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase Christopher ...

  10. Plan Your Experiment

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    ...vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular...more likely to result in a non-functional protein product. Be aware that there could be downstream translation...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...

  11. Zhang Lab CRISPR Page

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    ...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop...

  12. Fluorescent Protein Guide: Empty Backbones

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    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and quantum yield divided...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...

  13. CRISPR Guide

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    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147...

  14. Fluorescent Protein Guide: Biosensors

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    ... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005...
Showing: 1 - 14 of 14 results