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We narrowed to 15 results for: lentiviral production

Showing: 1 - 15 of 15 results

  1. Viral Production

    Type
    Collection
    ...Service Viral Production Viral Production Jump To: Viral Production Overview AAV Production Titer Quality...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is... to generate high quality preparations. After production, all virus preps are titered and subjected to...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...fusion protein. This means that the final product should be translated as a single string of amino...N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...N-terminal fusions pDEST Gateway vectors - For the production of His6-MBP-tagged fusion proteins in E. coli...

  3. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...modifications may enhance infectious particle production, including codon optimization, deletion of the...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly...Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase...pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP. Neville ... Sanjana 164432 pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase...

  4. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...genetic information to cells. These vectors rely on production plasmids that have been carefully optimized for...different types of viral vectors and find viral production and transfer plasmids for a wide array of research...Infected Host Genome Integration Immune Response Lentiviral Stable RNA < 8 kb 80–130 nm Dividing/Non-dividing...vitro cell cultures, these plasmids direct the production of viral vectors that contain the desired genetic...

  5. Kazuhiro Oka Lentiviral Vectors

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    ...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid ...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  6. Lentivirus Plasmids

    Type
    Collection
    ...generations, viral production, and viral safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations...Looking for lentiviral plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...Addgene lentiviral packaging, envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...for safe use in the lab. One safety feature of lentiviral vectors is that the components necessary to produce...

  7. Adenovirus Plasmids

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    Collection
    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...expression cassette. Bunz 16402 pShuttle Shuttle For production of viruses containing transgene under a chosen...promoter Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter...promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene...promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene... plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene...under CMV Bamburg 50958 ShuttleNSE Shuttle For production of viruses containing transgene under Neuron-...

  8. Luciferase Plasmid Collection

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    Collection
    ...interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla...21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase Eric Campeau... pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau... 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase Christopher ...

  9. Plan Your Experiment

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    ...vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular...more likely to result in a non-functional protein product. Be aware that there could be downstream translation...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...

  10. Zhang Lab CRISPR Page

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    Collection
    ...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...complex with a 2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop...

  11. Fluorescent Protein Guide: Empty Backbones

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    ...Snapp . Note for all tables: Brightness is the product of exctinction coefficient and quantum yield divided...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...Mammalian Expression pLenti6.2_miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression...

  12. COVID-19 Resources

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    Collection
    ...membrane protein, M), including plasmids for protein production, secretion, and codon-optimized ... lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee...

  13. Viral Prep Service for Plasmids and Pooled Libraries

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    ...about Packaged on Request Production and Quality Control Production Our production process and quality control...pricing includes DNA amplification, viral vector production, purification, and comprehensive quality control...Articles Additional details about viral vector production at Addgene, ordering information, and more. Additional... libraries. Viral preparation options include lentivirus and AAV. Viral...immediately after MTA approval. Browse AAV Browse Lentivirus Packaged on Request Choose from 30,000+ options...

  14. CRISPR Guide

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    ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147...

  15. Fluorescent Protein Guide: Biosensors

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    ... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005...
Showing: 1 - 15 of 15 results