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We narrowed to 14 results for: mouse c rel

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Showing: 1 - 14 of 14 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...246-54. Dowdy Adenovirus Mouse Non-integrating expression of mouse Sox2, Oct4, c-Myc, and Klf4 from four...Hochedlinger Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4, and c-Myc from four separate...Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, Sox2, Klf4, and c-Myc from ...for generating mouse iPS cells; retroviral expression of mouse Sox2, Oct3/4, Klf4, and c-Myc from separate...Woltjen Plasmid Mouse Non-integrating polycistronic expression of mouse Oct4, Klf4, Sox2, and c-Myc from two...episome Mouse Non-integrating EBNA1-mediated polycistronic expression of mouse Oct4, Sox2, Klf4, c-Myc, ...reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9. Jaenisch Lentivirus Mouse Polycistronic...

  2. Rett Syndrome

    Type
    Collection
    ...M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (M6818) mouse antibody...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...top Animal Models Studies of the MECP2 protein in mouse and rat models have accelerated our understanding...Search (Link opens in a new window) JAX Mice for mouse models that include MECP2 null alleles or common...following the link to their laboratory website. Mouse Line Mutation (DNA) Background Strain Purpose Publication...endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) ...Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion of Xist Unpublished...

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a...Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin/GRP75 [N419/40R] Mortalin/GRP75 Mouse Mouse IgG2a 114549...Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin-1, 11NQ [N76/3R] Ataxin-1, 11NQ Mouse Mouse IgG2a 114560...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a.../130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [L113/13R] Homer1L Mouse Mouse IgG2a 177458 Anti-Homer1L...[L131/17R] SPHKAP Mouse Mouse IgG2a 177478 Anti-SPHKAP [L131/20R] SPHKAP Mouse Mouse IgG2a 177479 Anti-SPHKAP...K+ channel Mouse Mouse IgG2a 177497 Anti-Thorase/Atad1 [N125/10R] Thorase/Atad1 Mouse Mouse IgG2a 177498...

  4. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...re-engineer conditional alleles in mouse embryonic stem cells. Return to top Related Addgene Plasmid Collections...be toxic in some systems, such as Drosophila and mouse, particularly in proliferating cells. Expression...Libraries Pooled Library Name Type PI Description Mouse Tumor Suppressor Gene CRISPR Knockout Library CRISPR...Cre to interrogate tumorigenesis in vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name... a circular piece of DNA (and is not maintained). C) If the sites are on separate DNA molecules (in trans...proteins: The recombinase is split into inactive N- and C-terminal fragments and placed under the control of...

  5. Plan Your Experiment

    Type
    Collection
    ...Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann, M., &...enables researchers to manipulate the genome with relative ease. Over the past decade, researchers have expanded...method is a common choice for generating modified mouse lines. Protein delivery Lastly, you can deliver ... edit through HDR. Base and prime editing are relatively inefficient methods and often require screening...Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome...Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...25408407 Dixit, A., Parnas, O., Li, B., Chen, J., Fulco, C. P., Jerby-Arnon, L., Marjanovic, N. D., Dionne, D...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck DA, Deisseroth K...Navarro M, Burnham N, Cristiano C, Dorrier CE, Tipton GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele...Markovic M, Wolff SB, Ramakrishnan C, Fenno L, Deisseroth K, Herry C, Arber S, Lüthi A. 2016. Midbrain ...including dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains a public ... in order to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre...processing, producing a functional molecular tool (C,F). Implementation The following resources may be ...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...opens in a new window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives...transduced cells express both components in the same relative ratio. Different generations of transactivators...of background expression. For example, only a relatively low level of doxycycline can access the brain...toxicity, a known half-life (24 hours), and is relatively inexpensive. The induction profiles for dox and...20321 TetO-FUW-OSKM Tet-On inducible expression of mouse Oct4, Sox2, Klf4, and Myc for iPS cell generation...vector to express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 ... N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral ...

  8. COVID-19 Resources

    Type
    Collection
    ...Nucleocapsid Protein [mBG17] Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...Nucleocapsid Protein [mBG86] Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-CoV-2 nucleocapsid...Andersen, K. G., Rambaut, A., Lipkin, W. I., Holmes, E. C., & Garry, R. F. (2020). The proximal origin of SARS-CoV..., Wu, N. H., Nitsche, A., Müller, M. A., Drosten, C., & Pöhlmann, S. (2020). SARS-CoV-2 Cell Entry Depends...the knowledge and understanding of COVID-19 and related coronaviruses. Addgene assisted this effort by ...tools that are useful for COVID-19 related research. Additionally, we have linked to collections...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Antibodies...

  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pTag-RFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc...-RFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Endosomes...pCMV-mGold2t-CAF1-C-10 Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-C-10 Nucleus CAF...pCMV-mGold2t-Actin-C-18 Actin filaments beta-Actin mGold2t Francois St-Pierre 231772 pCMV-mGold2s-Actin-C-18 Actin...pCMV-mGold2t-Tubulin-C-18 Microtubules alpha-Tubulin mGold2t Francois St-Pierre 231771 pCMV-mGold2s-Tubulin-C-18 Microtubules...pCMV-mGold2t-Rab4a-C-7 Endosomes Rab4a mGold2t Francois St-Pierre 231773 pCMV-mGold2s-Rab4a-C-7 Endosomes Rab4a...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...CCL1 chemokine (C-C motif) ligand 1 I-309, P500, SCYA1, SISe, TCA3 CCL11 chemokine (C-C motif) ligand 11...chemokine (C-C motif) ligand 13 CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, SCYL1 CCL14 chemokine (C-C motif...chemokine (C-C motif) ligand 19 CKb11, ELC, MGC34433, MIP-3b, MIP3B, SCYA19 CCL2 chemokine (C-C motif) ligand...chemokine (C-C motif) ligand 20 CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38 CCL21 chemokine (C-C motif) ligand...CCL24 chemokine (C-C motif) ligand 24 Ckb-6, MPIF-2, MPIF2, SCYA24 CCL25 chemokine (C-C motif) ligand 25...chemokine (C-C motif) ligand 26 IMAC, MGC126714, MIP-4a, MIP-4alpha, SCYA26, TSC-1 CCL27 chemokine (C-C motif...CCL28 chemokine (C-C motif) ligand 28 CCK1, MEC, MGC71902, SCYA28 CCL3 chemokine (C-C motif) ligand 3 ...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces...and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish...Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero ...23287718 Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-Based...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver...intracellular or extracellular glucose in vivo in mouse brain. Tim Brown , HHMI-JRC Tool Translation Team...membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. eLife. 2020 Jun 9;9. pii: 56469...GMP) FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-...Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010...Plasmids Joel Kralj Voltage Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange...sensor (MARIO) A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...LRRK2 C-His LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40376 pBACgus/LRRK2 1867-2141 C-His...2161 C-His LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40378 pBACgus/LRRK2 1867-2176 C-His...2161 C-His_T2031E LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40381 pBACgus/LRRK2 C-His_G2019S...2161 C-His_G2019S LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40383 pBACgus/LRRK2 C-His_C2024S...mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's, FTD Michael Davidson 55311 mTagBFP2-MAPTau-C-10 MAPT mTagBFP2... mKO2-MAPTau-C-10 MAPT mKO2 CMV Parkinson's, FTD Michael Davidson 58112 tdTomato-MAPTau-C-10 MAPT tdTomato...CMV ALS Alicia Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221...

  14. Genetic Code Expansion

    Type
    Collection
    .... coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase...C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase...coli azi-donorleucine (Anl) Bacterial ATG David Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli...musculus azidonorleucine (Anl) Mammalian ATG David Tirrell 91705 pSupAR-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers...using genetic code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3)... by tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair ...
Showing: 1 - 14 of 14 results