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Showing: 1 - 13 of 13 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...246-54. Dowdy Adenovirus Mouse Non-integrating expression of mouse Sox2, Oct4, c-Myc, and Klf4 from four...Hochedlinger Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4, and c-Myc from four separate...Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, Sox2, Klf4, and c-Myc from ...for generating mouse iPS cells; retroviral expression of mouse Sox2, Oct3/4, Klf4, and c-Myc from separate...Woltjen Plasmid Mouse Non-integrating polycistronic expression of mouse Oct4, Klf4, Sox2, and c-Myc from two...episome Mouse Non-integrating EBNA1-mediated polycistronic expression of mouse Oct4, Sox2, Klf4, c-Myc, ...reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9. Jaenisch Lentivirus Mouse Polycistronic...

  2. Rett Syndrome

    Type
    Collection
    ...M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (M6818) mouse antibody...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...top Animal Models Studies of the MECP2 protein in mouse and rat models have accelerated our understanding...Search (Link opens in a new window) JAX Mice for mouse models that include MECP2 null alleles or common...following the link to their laboratory website. Mouse Line Mutation (DNA) Background Strain Purpose Publication...endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) ...Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion of Xist Unpublished...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... 10 11,750 Mouse Cherry Brie Pooled Library 170511 Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin ...130,209 Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide...Knockout Mouse Birsoy 3rd 8 22,909 Mouse Liver CRISPR Knockout Library 192824 Knockout Mouse Knouse 3rd...~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis...123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse Superti-Furga...Knockout Mouse Chen N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat ...Knockout Mouse Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a...Mitofusin-2 Mouse Mouse IgG2a 114548 Anti-Mortalin/GRP75 [N419/40R] Mortalin/GRP75 Mouse Mouse IgG2a 114549...Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin-1, 11NQ [N76/3R] Ataxin-1, 11NQ Mouse Mouse IgG2a 114560...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a.../130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [L113/13R] Homer1L Mouse Mouse IgG2a 177458 Anti-Homer1L...[L131/17R] SPHKAP Mouse Mouse IgG2a 177478 Anti-SPHKAP [L131/20R] SPHKAP Mouse Mouse IgG2a 177479 Anti-SPHKAP...K+ channel Mouse Mouse IgG2a 177497 Anti-Thorase/Atad1 [N125/10R] Thorase/Atad1 Mouse Mouse IgG2a 177498...

  5. Cre-lox system

    Type
    Collection
    ...Cre (CT#644) Cre; expression in developing limb mouse Prx1 Bacterial Tabin 14796 pClusterin-ERT2CreERT2... construct CMV Mammalian Hughes 40591 hGFAP-Cre mouse astrocyte expression of Cre hGFAP Mammalian Messing...fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac enhancer and promoter fragment minimal...Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre...inducible. Expressed in limb connective tissue mouse Prrx1 Vertebrate Tanaka 111151 Col1A2:TFPnls-T2a-mErt-Cre-Ert... Tamoxifen inducible. Expressed in fibroblasts mouse Col1A2 Vertebrate Tanaka 111152 Col2a1:TFPnls-mErt-Cre-Ert...Eisenhoffer 131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta ...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...: 95 °C for 15 min, 35 cycles of (95 °C for 30 sec, 60 °C for 1 min, 72 °C for 1 min), and 72 °C for 10...following parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp down to 25 °C at 5 °C/min. Dilute oligos...parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min); Cycle 21 (80 °C for 20 min). These cycling ...Incubate at 30 - 37 °C for 24 - 72 hr. 30 °C may enhance genome editing efficiency, but 37 °C is acceptable....cells to incubate at 37 °C for 3 - 7 days and allow the clones to incubate at 37 °C for 7 - 14 days. Vary...thermocycler and run the following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the DNA...clones. Run sample in thermocycler: 65 °C for 6 min and 98 °C for 2 min to extract gDNA. Screen each clone...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck DA, Deisseroth K...Navarro M, Burnham N, Cristiano C, Dorrier CE, Tipton GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele...Markovic M, Wolff SB, Ramakrishnan C, Fenno L, Deisseroth K, Herry C, Arber S, Lüthi A. 2016. Midbrain ...including dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains a public ... in order to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre...processing, producing a functional molecular tool (C,F). Implementation The following resources may be ...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...for Tet transregulators. Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546...3 is a rtTA version tTA Off Hsiao 34856 fos-tTA Mouse fos promoter plus part of the first intron (-764...developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion protein 16), an essential...encoded tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . 1994;48:345-69. PubMed . Transcriptional...cells. Yao F, Svensjö T, Winkler T, Lu M, Eriksson C, Eriksson E. Hum Gene Ther . 1998 Sep 1;9(13):1939...biomedical research. Bockamp E, Maringer M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel B. Physiol...rTetR, which reversed the phenotype and created a reliance on the presence of tetracycline for induction,...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...opens in a new window) A collection of neurological mouse models including complex and monogenic diseases....aggregation. Saha et al. Nat Commun. 2023 Feb 2. Study the C terminal domain of TDP-43 to better understand its... atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...diagnostics and treatments for Alzheimer’s disease and related disorders. The site curates information on useful...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces...and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish...Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, Romero ...23287718 Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Côté J, Doyon Y. 2015. A Scalable Genome-Editing-Based...Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann ...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ...CCL1 chemokine (C-C motif) ligand 1 I-309, P500, SCYA1, SISe, TCA3 CCL11 chemokine (C-C motif) ligand 11...chemokine (C-C motif) ligand 13 CKb10, MCP-4, MGC17134, NCC-1, NCC1, SCYA13, SCYL1 CCL14 chemokine (C-C motif...chemokine (C-C motif) ligand 19 CKb11, ELC, MGC34433, MIP-3b, MIP3B, SCYA19 CCL2 chemokine (C-C motif) ligand...chemokine (C-C motif) ligand 20 CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38 CCL21 chemokine (C-C motif) ligand...CCL24 chemokine (C-C motif) ligand 24 Ckb-6, MPIF-2, MPIF2, SCYA24 CCL25 chemokine (C-C motif) ligand 25...chemokine (C-C motif) ligand 26 IMAC, MGC126714, MIP-4a, MIP-4alpha, SCYA26, TSC-1 CCL27 chemokine (C-C motif...CCL28 chemokine (C-C motif) ligand 28 CCK1, MEC, MGC71902, SCYA28 CCL3 chemokine (C-C motif) ligand 3 ...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...LRRK2 C-His LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40376 pBACgus/LRRK2 1867-2141 C-His...2161 C-His LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40378 pBACgus/LRRK2 1867-2176 C-His...2161 C-His_T2031E LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40381 pBACgus/LRRK2 C-His_G2019S...2161 C-His_G2019S LRRK2 His polH Parkinson's Michael J Fox Foundation MJFF 40383 pBACgus/LRRK2 C-His_C2024S...mCherry-MAPTau-C-10 MAPT mCherry CMV Parkinson's, FTD Michael Davidson 55311 mTagBFP2-MAPTau-C-10 MAPT mTagBFP2... mKO2-MAPTau-C-10 MAPT mKO2 CMV Parkinson's, FTD Michael Davidson 58112 tdTomato-MAPTau-C-10 MAPT tdTomato...CMV ALS Alicia Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver...membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. eLife. 2020 Jun 9;9. pii: 56469...GMP) FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-...Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010...Plasmids Joel Kralj Voltage Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...
Showing: 1 - 13 of 13 results