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  1. Control AAV Preps

    Type
    Collection
    ...50465 pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Roth 50469 pAAV-CaMKIIa-EGFP CaMKIIa...-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent...131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent 1, 5 Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn ...hSyn EGFP Cre dependent 1, 2, 5, 8, rg* Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1, 2,...Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive 8 Deisseroth 131001 pAAV-hSyn-mScarlet Syn...135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP Constitutive 1, 9, PHP.eB Dimidschstein 137135 pAAV-Ef1a-oScarlet... 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent 1, 2, ...

  2. Retrograde AAV viral preps

    Type
    Collection
    ...AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Roth...Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Roth 114472 pAAV-hSyn-mCherry Syn mCherry Control Deisseroth...Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 50457 pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent... 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth 50474 pAAV-hSyn-hM3D(Gq...DREADD Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA...Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Deisseroth 50459 pAAV-hSyn-DIO-mCherry Syn ...Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth 28306 pAAV-FLEX-tdTomato...

  3. Chemogenetics AAV Preps

    Type
    Collection
    ...IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4...Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry...Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry...Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor...Inhibition IRES mCitrine Cre-dependent 8 Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation... and IRES mCitrine Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition... and IRES mCitrine Cre-dependent 8 Roth 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry...

  4. Brain Initiative Collection

    Type
    Collection
    ...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD ... coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An ...PSAM4 GlyR IRES EGFP Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses...expression using the promoter hSyn Francois St-Pierre 179464-AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded...expression using the promoter hSyn Francois St-Pierre 198513-AAV1 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized...with EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...

  5. Biosensor AAV Preps

    Type
    Collection
    ...9 Li 113050 pAAV-hSyn-GRAB_DA1h Syn GRAB_DA1h none Constitutive 9, rg* Li 140553 pAAV-hsyn-GRAB_DA2m Syn...Constitutive 9 Li 140554 pAAV-hsyn-GRAB_DA2h Syn GRAB_DA2h none Constitutive 9 Li 140555 pAAV-hsyn-GRAB_DA-mut Syn... 9 Li 140556 pAAV-hsyn-GRAB_rDA1m Syn GRAB_rDA1m none Constitutive 9 Li 140557 pAAV-hsyn-GRAB_rDA1h Syn...140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208698 pAAV-hSyn-GRAB-gDA3m...Sensors 123308 pAAV-hSyn-GRAB_NE1m Syn GRAB_NE1m none Constitutive 9, rg* Li 123309 pAAV-hSyn-GRAB_NE1h Syn...Sensors 185386 pAAV-hSyn-GRAB_OT1.0 Syn GRAB_OT1.0 none Constitutive 1, 9 Li 185388 pAAV-hSyn-GRAB_OTmut ... 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Boyden 115892 pAAV-Syn-Archon1...

  6. Caltech Systemic Capsids

    Type
    Collection
    ... Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth ... 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D...Optogenetics 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2...Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible...105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control...Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP...Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell...

  7. Optogenetics AAV Preps

    Type
    Collection
    ...26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Deisseroth 26972 pAAV-hSyn-eNpHR...Deisseroth 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Boyden 84445 pAAV-CAG-FLEX-rc...pAAV_hSyn-PdCO-EGFP-WPRE Syn PdCO EGFP Constitutive 1, 5 Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE EF1a...202199 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE Syn PdCO, ChrimsonR (soma-targeted) EGFP Cre dependent...83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Fishell 135634 pAAV-S5E2...137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-... 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Deisseroth 137163 pAAV-nEF-Con...

  8. Cre-lox system

    Type
    Collection
    ...backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression hSyn AAV Zhang...neurons aCamKII AAV Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Cre and dTomato hSyn AAV Larsen 107787 AAV.TBG.PI.Cre.rBG...EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha Mammalian Sauer 11955 pBS505 EF1alpha-EGFPcre* Cre-EGFP fusion...105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion hSyn AAV Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40...Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Cre hSyn AAV Wilson 105555 pENN.AAV.hSyn.Cre.hGH Cre hSyn AAV Wilson...alpha AAV Deisseroth 55635 pAAV-EF1a-sCre SCre EF-1 alpha AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha...Retroviral Luikart 67503 pF CAG luc-EGFP-cre puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer ...

  9. Recombinases AAV Preps

    Type
    Collection
    ...9 Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn ... Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...AAV phSyn1(S)-FlpO-bGHpA Syn none rg* Zeng 174378 pAAV-syn-Flpo Syn none rg* Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo...) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a none rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1...rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Cepko 69571 pAAV-EF1a-C-CreintG EF1a none ... 5, 8, 9, rg* Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* ...Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-1-26973P 26973-AAV1 pAAV-hSyn-hChR2(...AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-5-26972P 26972-AAV5 pAAV-hSyn-eNpHR...26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-5-26975 26975-AAV5 pAAV-CaMKIIa-hChR2... 59171-AAV5 pAAV-Syn-ChrimsonR-tdT Optogenetics Ed Boyden AV-5-PV3638 65014-AAV5 pAAV-hsyn-Jaws-KGC-GFP-ER2...-AAV9 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-9-26973P 26973-AAV9 pAAV-hSyn-hChR2(...PV3637 65015-AAV8 pAAV-CaMKII-Jaws-KGC-GFP-ER2 Ed Boyden AV-9-26972P 26972-AAV9 pAAV-hSyn-eNpHR 3.0-EYFP ...26972P 26972-AAV1 pAAV-hSyn-eNpHR 3.0-EYFP Karl Deisseroth AV-8-PV3706 62726-AAV8 pAAV-Syn-Chronos-tdTomato...

  11. AAV Molecular Tools

    Type
    Collection
    ...tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a 5,...transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent...Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the...synaptophysin-mRuby for labeling of axon terminals. 1 Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent...Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...

  12. Serotype Testing AAV

    Type
    Collection
    ...Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Chiba 185714 pAAV-hSyn-GFP-IRES-mSNCA SNCA GFP hSyn Parkinson's Jun Ding 185715 pAAV-hSyn-GFP-IRES-hSNCA...185717 pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P) SNCA GFP hSyn Parkinson's Jun Ding 185718 pAAV-hSyn-GFP-IRES-hSNCA... 107934 pAAV-hSyn-Htt171-66Q-myc-WPRE HTT hSyn1 Huntington's Johan Jakobsson 107936 pAAV-hSyn-Htt171-18Q-myc-WPRE...pAAV-hSyn-GFP-IRES-hSNCA SNCA GFP hSyn Parkinson's Jun Ding 185716 pAAV-hSyn-GFP-IRES-mSNCA(1-95) SNCA GFP hSyn Parkinson's ...) SNCA GFP hSyn Parkinson's Jun Ding 185719 pAAV-hSyn-GFP-IRES-hSNCA (S129D) SNCA GFP hSyn Parkinson's...pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1 SOD1 H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1...14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ...recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...sequence (20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables...: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This ...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A ...SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro...) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single guide RNA 62987 : PX462...

  15. Brain Armamentarium

    Type
    Collection
    ...AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal...AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal...AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal...Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825...Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3...Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias:...Jonathan Ting Viviana Gradinaru 214856-PHPeB AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias:...
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