We narrowed to 16 results for: plasmid cloning by pcr

...For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The ...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...recombinase and an sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which...2A-EGFP-KASH and an sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two... used in the SpCas9 plasmid series. ), and a pair of annealed oligos can be cloned scarlessly into the...

... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled...

...Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall and Myers ...arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall and Myers Tagging Plasmids Protein...backbone to clone the left and right homology arms. More details on how to use this plasmid in your experiments...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...

...systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired...oligos into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However, ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based methods like...including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating...Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery Validate Genetic...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression....Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description... 2, 3, or 4. For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression...putative enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat...TALE Toolbox 49622 - 49647 24 plasmids Thoru Pederson TALEColor plasmids: destination vectors for use ...

...Synthetic Biology Plasmids MoClo Guide Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Blog: Golden Gate...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ... (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection contain genetic ...not functional by themselves. Researchers should clone these enzymes into an expression vector of their...

...gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection... Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning All-in-one ...All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6...

... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...expression - Gradia Lab plasmids for mammalian expression, Ligation Independent Cloning (LIC) pAG CMV Empty...Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses...expression - Gradia Lab plasmids for bacterial expression, Ligation Independent Cloning (LIC) Also see our ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits...Kits are sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore...

...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...search for popular empty backbone plasmids into which you can clone your regulatory element or gene of...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid...

...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...type, species, and more. Plasmids Browse the table below to see all of the plasmids that are currently available...Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create...Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. ...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...

...DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the specific... Plasmid Collections Zinc Finger Consortium Zinc Finger ...cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536 OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc...

...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR Plasmids page ...Guide . Browse CRISPR plasmids available at Addgene through our CRISPR Plasmids and Resources page. Check...

...anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved ...isolate clones containing the desired edit (see: Validate genetic modification ). Browse Plasmids: Double-Strand...the genomic DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...partner labs. Learn More scFv Plasmid Collection The following plasmids can be used to produce scFvs from... Pan-GRK Mouse Mouse Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies...research. Find plasmids encoding these R-mAbs here. Antibody...