...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...antibody production methods and quality control processes for purified recombinant antibodies. Antibody...

...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic tools . pcDNA3.1+-...used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0 Firefly/Renilla...

...extraction solution, runs the protocol, then uses around 2-4ul in each 50ul PCR reaction. The gDNA extracted ...facilitate the processing of the crRNA) is also expressed from a separate promoter. In the pre-processing form,...construct from our 'Split RNA' design, where the pre-processing form of crRNA is expressed and a tracrRNA (the...in cell and with Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter form that contains...our 'Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically to a mature...

...dual-transgenic recombinase-expressing mouse lines 2-9 and combinations of transgenic recombinase-expressing... present, the introns are excised during mRNA processing, producing a functional molecular tool (C,F)....Deisseroth Lab maintains a Standard Operating Procedure with general principles for working with INTRSECT...