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We narrowed to 16 results for: retroviral vectors

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Showing: 1 - 16 of 16 results

  1. Retrovirus Plasmids

    Type
    Collection
    ... gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral Vector Guide for...about gamma-retroviral components, viral production, and viral safety. Gamma-Retroviral Vector Guide Popular...Additional Resources Gamma-Retroviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource...types. Wild-type gamma-retroviruses have been engineered into gamma-retroviral vectors for safe use in the... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging ...Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect dividing...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression...Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, ...transcriptional activating fusions in single lentiviral vectors for human iPSC generation OCT4 and SOX2 Work as...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, ...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of ...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...rtTA-Advanced Rudolf Jaenisch 25434 pMA2640 Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin... Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre...Schiedlmeier, B. (2011). Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral... lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin resistance...pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP. Neville Sanjana 164432...pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. Alejandro...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...

  9. CRISPR References and Information

    Type
    Collection
    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B)...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag...or search our complete collection and filter by vector type, popularity, and more. Blue/UV Protein Excitation...93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow Protein...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells... Mitophagy Dual-emission mitophagy biosensor (retroviral expression of pH sensitive mt-mKeima) The ubiquitin...mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging PI Return to ...

  12. Lentivirus Plasmids

    Type
    Collection
    ...engineered into viral vectors for safe use in the lab. One safety feature of lentiviral vectors is that the components...plasmids available at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components... viral production, and viral safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking...Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector system. Expresses gag-pol and rev . Garry Nolan...Kasparov Return to top Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled...Pooled Libraries All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource Guide Addgene blog: Tips for...Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common and useful types...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ... open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...CARMA1, MGC133069 CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence C-CBL, CBL2, RNF55 CBLB...CBLB Cas-Br-M (murine) ecotropic retroviral transforming sequence b DKFZp686J10223, DKFZp779A0729, DKFZp779F1443...Nbla00127, RNF56 CBLC Cas-Br-M (murine) ecotropic retroviral transforming sequence c CBL-3, CBL-SL, RNF57 ...Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors from the labs of William Hahn and David Root . ...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...

  14. Plasmid Collections

    Type
    Collection
    ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated Topics ...

  15. CRISPR Guide

    Type
    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with a ...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...HiBiT-TREM2 Vector TREM2 HiBiT CMV Alzheimer's Promega Corporation 238681 NanoLuc-TREM2 Vector TREM2 NanoLuc...HaloTag-TREM2 Vector TREM2 HaloTag CMV Alzheimer's Promega Corporation 238706 NanoBRET Assay Vector, CSF1R-NanoLuc...PINK1-SmBiT Vector PINK1 SmBiT CMV Parkinson's Promega Corporation 238783 PINK1-LgBiT Vector PINK1 LgBiT...LgBiT-TREM2 Vector TREM2 LgBiT CMV Alzheimer's Promega Corporation 238801 TREM2-SmBiT Vector TREM2 SmBiT...LgBiT Vector TREM2 LgBiT CMV Alzheimer's Promega Corporation 238850 EIF2AK2-NanoLuc Fusion Vector EIF2AK2...Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter CMV T7 polH GAL...
Showing: 1 - 16 of 16 results