...May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization... has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human ...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human ...Institute for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell... hiPS cell lines gRNA sequences used in the creation of the hiPS cell lines The following plasmids can...structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin...

...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over...Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic ...for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab. .... A useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in ...latest reference sequence assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated... curated database of genetic, genomic, and developmental data for zebrafish biology. Zebrafish International...

...See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...huntingtin proteins with abnormally long polyglutamine sequences. These glutamine-rich sequences are prone... iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders...misfolding and aggregation and can interfere with protein-protein interactions and nerve cell function within...curing and treating them. Scientists are studying protein aggregation, learning about the blood brain barrier...will advance more quickly with the avid sharing of data, tools, resources, methods, and results that are...modification and target species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...

...along with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA)...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461;...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended...

... includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring behavioral and ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...worm ( C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene...ordered individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology....Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

...step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the... (SARS classic) sequences. Mammalian Targets : Plasmids containing mammalian sequences linked to SARS-...SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...CoV, depends on binding of the viral spike (S) protein to cellular receptors. The cellular receptor for...antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available...

... domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2,...morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic protein 7 OP...TYROBP TYRO protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I...assay data for translational and clinical research. Sci Data. Feb 27;5:180015. doi: 10.1038/sdata.2018.15... You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...shock 70kDa protein 1B FLJ54328, HSP70-1B, HSP70-2, HSPA1A HSPA1L heat shock 70kDa protein 1-like HSP70...