...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's local concentration near ...

...is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells,... separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...inactive dCas9 fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-... complex through interactions between the gRNA scaffold and surface-exposed positively-charged grooves...or co-expression of dCas9-VP64 with a modified scaffold gRNA and additional RNA-binding helper activators...activators have been developed for bacteria by using a scaffold RNA that contains the gRNA and an RNA hairpin ...