We narrowed to 6 results for: cre dependent fluorescent reporter

...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA...have also led to reduced dependency on specific PAM sequences and the creation of new tools. Nickase and...binding signal for Cas9, but the exact sequence depends on which Cas protein you use, and there are several...homology arms). The length of each homology arm depends on the size of the change being introduced; larger...larger insertions require longer homology arms. Depending on the application, the donor DNA may be a single-stranded...

...chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific Cre is provided...luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs with increased efficiencies...excite or inhibit the neurons expressing the LMO, depending on its biophysical properties. While LMOs provide...efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native coelenterazine...ON or turn OFF the neurons in your experiment? Depending on your answer, you'd pick a chemogenetic activator...EF1a, CAG General expression Targeted Expression Depending on the experiment, an AAV-encoded chemogenetic... FLEx vectors block expression in the absence of Cre and are especially useful in AAV experiments where...

...Plasmids , Cre-lox and Other Recombinases , TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids...the control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...cell types (mammalian, yeast, bacterial, etc.), depending largely on which promoter is used to initiate ... of genetic elements. These plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout...level of transcription driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral ...relies on the random incorporation of modified, fluorescently-tagged bases during in vitro DNA replication... DNA because the ultimate goal is to have a fluorescently-tagged nucleotide at each position in the DNA...

...of proteins) and sensors (genetically-encoded reporters of molecular signals). These areas have each since...turn ON or turn OFF neurons in your experiment? Depending on your answer, you'd pick an excitatory or inhibitory...results in more localized expression of the opsin. Depending on the virus and promoter system used, there is...combined with site specific recombinase technology like Cre-lox, which allows you to express an opsin ONLY in...using a viral vector with a floxed opsin in a VGAT-cre animal will result in expression of the opsin only...Alternatively, when one half of a given protein, such as the Cre recombinase, is fused to Cry2 and the other half ...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...

...not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...VSV-G is often put under an inducible promoter. Depending on the cell type, there are other envelope gene...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...scientists have made changes to these components for increased safety when producing viral vectors in a laboratory.... Figure 1: Wild-type gamma-retrovirus genome. Created with BioRender.com. All gamma-retroviral vectors...distinct plasmids for safety to reduce the chance of creating replication-competent retroviruses. For a summary...tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com. Gamma-Retroviral Vector Production...

...not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...from the LTR is Tat-dependent. Figure 3: Second-generation lentiviral plasmids. Created with BioRender.com...VSV-G is often put under an inducible promoter. Depending on the cell type, there are other envelope gene...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...transgene expression. Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing...removed or mutated for increased safety. Figure 1: Wild-type lentivirus genome. Created with BioRender.com...in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient way to determine what...