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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: ena/1000

Showing: 1 - 3 of 3 results

  1. Molecular Biology Reference

    Type
    Guide
    ...fluorescent signal. Sanger can sequence approximately 500-1000 bases downstream of the known primer region with...Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular components...the field of recombinant DNA technology, which enables the preparation of more managable (i.e., smaller...are relatively small (∼3000 - 6000 basepairs) to enable easy manipulation. Typically plasmids contain the...molecular cloning . The process of molecular cloning enabled scientists to break chromosomes down to study their...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion... purpose of replication. The selectable marker enables selection of a population of cells that have taken...

  2. Antibody Guide

    Type
    Guide
    ...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

  3. CRISPR Guide

    Type
    Guide
    ...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S.... on the same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic...family proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal...purification methods are more straightforward and enable direct identification of molecules associated with...editor Fusion of a Cas protein to a deaminase that enables direct base conversion in RNA or DNA without a ...2016). Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian ..., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., ...
Showing: 1 - 3 of 3 results