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We narrowed to 6 results for: plasmid transfection

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  1. Plan Your Experiment

    Type
    Guide
    ...For easy-to-transfect cell types (e.g. HEK293 cells), transfection with standard transfection reagents may...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...or gRNA in a mammalian cell line that can be transfected at high efficiency Lentiviral transduction Cas9...and enrich positive cells Packaging and envelope plasmids provide the necessary components to make lentiviral...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table... three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which ...as cis plasmid) — containing the transgene of interest between the two ITRs. Packaging plasmid (also known...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...possibility that recombination between the transfected plasmids could lead to the production of replication-competent...all of Addgene’s transfer plasmids are replication deficient. These plasmids can be used to create viral...

  3. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...that recombination between the transfected adenoviral transfer plasmid and the E1 region integrated in...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...backbone plasmid containing the adenoviral genes necessary for virus production. The shuttle plasmid and the...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...lentiviral plasmids (three or four) are transfected into HEK293T cells using your preferred transfection method...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or ...capacity Packaging plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ... The three plasmids described above (envelope, packaging, and transfer) are co-transfected into the HEK293T...on separate plasmids ( in trans ). This method, therefore, reduces the number of plasmids that are required...

  6. Antibody Guide

    Type
    Guide
    ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...
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