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We narrowed to 6 results for: plasmid transfection

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  1. Plan Your Experiment

    Type
    Guide
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...into mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...cells. These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...difficult to transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table... three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which ...as cis plasmid) — containing the transgene of interest between the two ITRs. Packaging plasmid (also known...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...possibility that recombination between the transfected plasmids could lead to the production of replication-competent...all of Addgene’s transfer plasmids are replication deficient. These plasmids can be used to create viral...

  3. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...that recombination between the transfected adenoviral transfer plasmid and the E1 region integrated in...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...backbone plasmid containing the adenoviral genes necessary for virus production. The shuttle plasmid and the...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...lentiviral plasmids (three or four) are transfected into HEK293T cells using your preferred transfection method...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or ...capacity Packaging plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ... The three plasmids described above (envelope, packaging, and transfer) are co-transfected into the HEK293T...on separate plasmids ( in trans ). This method, therefore, reduces the number of plasmids that are required...

  6. Antibody Guide

    Type
    Guide
    ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...
Showing: 1 - 6 of 6 results