We narrowed to 25 results for: ELP

... must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of...cells to detach. Gently tap the sides of the CS2 to help detach the cells, add 200 mL of DMEM complete media...μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2...Volume Needed: 582.1 μg / 1.0 μg/μL = 582.1 μL pHelper: 0.08 μg/bp × 11,854 bp = 949.8 μg Volume Needed...carefully tilting the CS5 back and forth. Pro-Tip To help distribute the media amongst the five layers, tilt...

...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...Placing saran wrap over the top of the flask can help with this, but is not necessary if you pay close...two purposes: 1) it provides a visible dye that helps with gel loading and allows you to gauge how far...

...agarose gel will help resolve smaller bands from each other, and a lower percentage gel will help separate larger...purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands. ...

...primers typically anneal to the backbone and can help you verify the ends of the insert. Longer genes ...about the accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The...If the mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction For many...cross-contamination with other substances. They also help you transfer the material to your tube or container...then place it unfolded onto the balance. This will help you remove it from the scale later on as otherwise...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction A water...water bath is a piece of laboratory equipment that helps bring your materials to a particular temperature...and maintain the desired temperature. This also helps the water bath heat up quicker. Pro-Tip Set up the...

...Protocols page . Please feel free to email us at help@addgene.org with any questions. Protocols Video ...Protocol How-To Videos Screencapture walk-throughs to help guide you through Addgene's online resources. (Link...frequently asked questions about the MTA process Help Center How to Deposit Your guide to depositing plasmids...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction The pipette...differently on each type of pipette. This section will help you understand how to read each display to dispense...action between the container and the liquid will help draw the liquid out of the pipette tip). Then, slowly...

...OR on dry ice for 5 min. Note: This freezing may help the DNA to precipitate. Centrifuge solution at high...pellet by adding 500 µL cold 70% ethanol. Note: This helps to remove excess salt from the DNA pellet. Centrifuge...supernatant after step 6 and before step 7. This will help to remove proteins and other contaminants from the...

...in your own work. Please feel free to email us at help@addgene.org with any questions. Intro to the Lab...the Lab Bench Introductory techniques designed to help you get started in the lab. Name Description (Link...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction Microscopes... from the light source through a condenser that helps concentrate the light onto the sample, and then ...

...not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that can both function...good idea to run an uncut sample of each vector to help with troubleshooting if your digests don’t look ...

...lysate. Pro-Tip Having your samples spin at 4°C helps reduce RNA degradation. If you don’t have access...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction There are...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction A centrifuge...

...before growing in liquid culture. More aeration may help to increase the density of the culture. Normally...

...Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction There are...

...that can be more easily transformed and that will help to maintain the plasmid without rearrangement of...

... clearly distinguish the steps. This method will help enrich the prep with full (genome containing) particles...

...good idea to run an uncut sample of each plasmid to help with troubleshooting if your digests don’t look ...