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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 46 results for: IGH@

Showing: 31 - 46 of 46 results

  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...platform. Pour off the water in the sink. Take a brightfield image of the gel with an appropriate imaging ...25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should ...

  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography...and gently pour off the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top...

  3. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...bases. This is an example of a trace file from a high-quality portion of a sequencing reaction: Sequence...file from a portion of a sequencing reaction with high background: If you obtain a different sequence than...

  4. Water Bath Protocol

    Type
    Protocol
    ...Distilled water Disinfectant Thermometer Water bath weights and floats Reagents None needed Procedure Ensure...be used to secure tubes in place and water bath weights can hold bottles in place. After putting your tubes...

  5. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...extinguisher Guidelines BSL-1 Guidelines Before You Work Right after entering the lab, put on the proper personal.... BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded and unloaded in ...

  7. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...Incubate at room temperature for 2hr, or at 16°C overnight (following the manufacturer’s instructions). Note...For many ligation reactions, especially if using "high concentration" ligase, 5min at room temperature ...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ...your plasmid and order primers (see figure to the right). When designing your plasmid, think about what ...fragments can subsequently anneal to each other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides...

  9. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ... 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure Section 1: Seeding cells The...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....appropriate PCR tubes. Pipette back and forth 5 times. Lightly cap the PCR tubes. Generate the Droplets Bring ...

  11. Kit Free RNA Extraction

    Type
    Protocol
    ...C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 minutes at 10,000 x g at 4°...

  15. Video Library

    Type
    Protocol
    ...Addgene's online resources. Career Videos Get career insight and advice from real Addgenies! Protocols (Link...
Showing: 31 - 46 of 46 results