Skip to main content
Addgene
Showing: 21 - 29 of 29 results

  2. Pouring LB Agar Plates

    Type
    Protocol
    ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

  3. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

  6. AAV ddPCR Titration

    Type
    Protocol
    ...The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where...

  7. CRISPR Library Amplification

    Type
    Protocol
    ... but care must be taken to perform adequate NGS based QC to ensure no change in representation compared...

  8. Lentivirus ddPCR Titration

    Type
    Protocol
    ...The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...assembly of DNA molecules up to several hundred kilobases. Nature Methods , 6(5), 343–345. https://doi.org...
Showing: 21 - 29 of 29 results