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We narrowed to 31 results for: Cel

Showing: 21 - 31 of 31 results

  1. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... an individual plasmid within a single bacterial cell (Figure 2). Large plasmids usually have a low copy...copy number (approximately one or two copies per cell) and they need to grow for longer periods of time... can be present in large numbers, 50 or more per cell, and have a high copy number. High copy number plasmids... increase this to 350–400 rpm to obtain a higher cell density. Resources Additional Protocols Streaking...

  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...after use. Carefully pour the filtered and diluted cell culture supernatant into the Gravitrap column. Collect...

  3. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria... and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can...

  4. Using a Light Microscope Protocol

    Type
    Protocol
    ...like microbes in a drop of pond water or the cells in your cell culture dish. Microscopes come in a huge ...transmission electron microscopes that allow us to see cellular components with science-fiction levels of detail...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...samples may not have been processed correctly in the cell (e.g., failure to cleave the signal peptide) and...Scatter chart. Change the data range and select the cells where the x-axis is represented. Add a linear trendline...

  6. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly..., iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient media...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified...

  9. Water Bath Protocol

    Type
    Protocol
    ...restriction digests needed in molecular biology, and thaw cell lines. There are many different types of water baths...
Showing: 21 - 31 of 31 results