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  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR and Ppurify the PCR Product Run PCR to...and recipient plasmid. Because you lose some DNA during the gel purification step, it is important to digest...

  3. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...for different cell types. Before starting this procedure, be sure that the cell pool has been sufficiently...well plate gave rise to 15–20 monoclonal lines. Procedure Generating Conditioned Medium (optional) Seed ...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials...samples on ice or pre-chilled 96-well freezer blocks during use. In this protocol, a dilution series is prepared...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the risk of contaminating reagents...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...HEK-293T cells that were inadvertently collected during harvesting. TIP: In lieu of centrifugation, you...

  7. DNA Quantification

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular cloning, ...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ... (with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate antibiotic...

  11. Water Bath Protocol

    Type
    Protocol
    ... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

  12. Ligation Independent Cloning

    Type
    Protocol
    ...annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for LIC typically...

  16. General Transfection

    Type
    Protocol
    ...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

  18. Gibson Assembly Protocol

    Type
    Protocol
    ...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...

  19. Immunocytochemistry

    Type
    Protocol
    ...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...
Showing: 21 - 40 of 46 results