We narrowed to 39 results for: Dos

Learn how to transform E. coli with your plasmid of interest.

Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette.

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ

Lab protocol for transfection of HEK293 cells to produce recombinant antibody.

Tips on designing primers for PCR

Protocol for creating a glycerol stock, including additional tips for long-term storage.

In this protocol you will learn the basics of how to weigh reagents.

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Learn how to add antibiotic to a plate after it's already been poured and solidified.

Find step-by-step instructions on how to use a water bath in the lab.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Learn about selecting and using a centrifuge to separate different components in a liquid sample.

In this protocol you will learn the basics of how to use a light microscope.

This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

Use this protocol to transfect mammalian cells with your plasmid of interest.

Use this protocol to generate lentivirus

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.