We narrowed to 29 results for: MLS

...20 in PBS) as follows: Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several...the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate...9018 Bovine serum albumin (BSA), VWR 10273-264 50 mL conical tubes, VWR 89039-656 96-well polyester (clear...150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518...1% BSA in PBS) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to ... BSA in PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix...starting with three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using...

...target plate, add between 0.5–1 mL virus (add 0.5 mL for a high MOI, and 0.1 mL for a low MOI). Scale the amount...#7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing Oligos Resuspend...concentration of puromycin should be from 1-10 μg/mL in 1 μg/mL increments. d. Label plates from 1-10 and add...1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL antibiotic. Pour...protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog...Inoculate 5 colonies from each ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down ...to be transfected, plate 7×105 HEK-293T cells in 5 mL of media in a 6 cm tissue culture plate. Incubate...

...g of agarose in 100 mL of TAE will make a 2% gel). Mix agarose powder with 100 mL 1xTAE in a microwavable...approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the...Agarose Ethidum bromide (stock concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure...buffer, place the gel into a container filled with 100 mL of TAE running buffer and 5 μL of EtBr, place on ...

...10 12 GC/mL to >2 x 10 13 GC/mL and we use an internal reference virus that is 1 x 10 13 GC/mL. We recommend...1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected to have a titer >3 x 10 13 GC/mL, use dilutions... add carrier DNA to a final concentration of 4 ug/mL to each standard dilution. Dilute DNase-treated and...the physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions...

...Sample Dilution Concentration Titer (GC/mL) Average Titer (GC/mL) AAV Sample 1 6400000 965 3.09E+13 3.2E...are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents... $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume (20 µL) C = Copies/µL D = Dilution...

...on your benchtop and typically hold the common 1.5 mL microfuge tubes used in labs, though some larger ...samples. For example, spinning down samples in 1.5 mL microfuge tubes at 10,000 rpm would require a tabletop...

...overnight culture to 500 μL of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes:...

... in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance...

...dispense small amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing...