We narrowed to 40 results for: NSI;

...and molecular biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction... Assembly master mix (isothermal reaction mix) consists of three different enzymes within a single buffer...

...linear polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell...discard the tube and thaw a new working stock. Considerations Before You Start The health of the cell line...

...well. Allow the HeLa cells to grow to the desired density before labeling. Section 2: Fixing and permeabilizing...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

... 96-well optical plate Pipette tips General Considerations Always run standards and samples in duplicate... #59462 is one plasmid that gave reliable and consistent results. Use the recommended plasmid, or test...

... bath Pipetman Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein...colonies will grow). However, it is also more expensive. Procedure Measure 37g of pre-mixed LB-agar powder...

...After staining with Coomassie blue, protein band intensities are measured using ImageJ software and a standard...similar photo software to determine the relative intensity of the protein bands to the overall lane. Import...

...blocking buffer and optimal antibody concentrations. Consider titrating your antibody to determine the optimal...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

... (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but this upfront cost...for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing LB containing bacteria...

...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

...for broader pairing capabilities. Taking into consideration the information above, primers should generally...

... experiments. There are a number of factors to consider when selecting sequencing primers, including: ...

... to test individual clones before use of more expensive forms of plasmid verification, such as DNA sequencing...

...Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...

...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

...The lowest efficiency cells (usually the least expensive) are fine for transforming plasmid DNA for the...

...batch of 1 mg/mL PEI and for each cell line. Considerations Before You Start The health of the packaging...

...high percentage of glycerol that increases the density of your DNA sample causing it settle to the bottom...

...Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the 5'...

...support reproducibility in science. General Considerations The ELISA can be completed in a single day ...

...suitable for handling HIV-derivative viruses. For transient knockdown of protein expression, you may transfect...